Cryopreserved ampule of Rat Microglia containing ≥ 2 million cells
Creative Biolabs rat microglia are cryopreserved ready-to-use high-quality cells prepared by standard methods and can be cultured immediately in supplemented DMEM. Each vial contains approximately 2 million cells in 0.25 ml of suspension.
Recommended medium: DMEM (NCC20-9PZ30) supplemented with heat-inactivated FBS, penicillin/streptomycin.
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased