Mouse beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1 (NM_011792) ORF clone/Lentiviral Particle, Untagged

Description

This is a mouse BACE1 untagged clone expression plasmid or lentiviral particle, ready for transfecting into mammalian cells.

Gene/Insert Name

BACE1

Species

Mouse

Insert Size (bp)

1506 bp

Tag

Tag Free

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Kanamycin (25 ug/mL)

Cell Selection

Neomycin

ORF Nucleotide Sequence

AGCCTGCCTAGGTGCTGGGAGCTGGGAGCTGGATTATGGTGGCCTGAGCAGCCGACGCAGCCGTAGGAGC CCGGAGTCCCTGTCGGTCCCCAAGCTGCAAAGCCCGCCTGGAAGACCCCGAAAGCTACGGGCTCGGATAG CCATGCCCGCCCCTCCCAGCCCCACAAGGGGCCCGATCCCCCCGCTGGCGGCCGGCGTCCAGATGTAGCT GGGTCCCCTGGATCGCCATCGTCGTCTCCTCTCGTGCGCTACGGATTTCTCCTGCCCACTCTCCGCCGCC TGGACCGGGAACTGAGCGAGGGGCCTGCAGACTCTGCAGTCCTGATGCCGCCGAGGCCGCTCTCCTGAGA GAAGCCACCACCACCCAGACTTAGGGGCAGGCAAGAGGGACAGTCACCAACCGGACCACAAGGCCCGGGC TCACTATGGCCCCAGCGCTGCACTGGCTCCTGCTATGGGTGGGCTCGGGAATGCTGCCTGCCCAGGGAAC CCATCTCGGCATCCGGCTGCCCCTTCGCAGCGGCCTGGCAGGGCCACCCCTGGGCCTGAGGCTGCCCCGG GAGACCGACGAGGAATCGGAGGAGCCTGGCCGGAGAGGCAGCTTTGTGGAGATGGTGGACAACCTGAGGG GAAAGTCCGGCCAGGGCTACTATGTGGAGATGACCGTAGGCAGCCCCCCACAGACGCTCAACATCCTGGT GGACACGGGCAGTAGTAACTTTGCAGTGGGGGCTGCCCCACACCCTTTCCTGCATCGCTACTACCAGAGG CAGCTGTCCAGCACATATCGAGACCTCCGAAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAGG GGGAACTGGGCACCGACCTGGTGAGCATCCCTCATGGCCCCAACGTCACTGTGCGTGCCAACATTGCTGC CATCACTGAATCGGACAAGTTCTTCATCAATGGTTCCAACTGGGAGGGCATCCTAGGGCTGGCCTATGCT GAGATTGCCAGGCCCGACGACTCTTTGGAGCCCTTCTTTGACTCCCTGGTGAAGCAGACCCACATTCCCA ACATCTTTTCCCTGCAGCTCTGTGGCGCTGGCTTCCCCCTCAACCAGACCGAGGCACTGGCCTCGGTGGG AGGGAGCATGATCATTGGTGGTATCGACCACTCGCTATACACGGGCAGTCTCTGGTACACACCCATCCGG CGGGAGTGGTATTATGAAGTGATCATTGTACGTGTGGAAATCAATGGTCAAGATCTCAAGATGGACTGCA AGGAGTACAACTACGACAAGAGCATTGTGGACAGTGGGACCACCAACCTTCGCTTGCCCAAGAAAGTATT TGAAGCTGCCGTCAAGTCCATCAAGGCAGCCTCCTCGACGGAGAAGTTCCCGGATGGCTTTTGGCTAGGG GAGCAGCTGGTGTGCTGGCAAGCAGGCACGACCCCTTGGAACATTTTCCCAGTCATTTCACTTTACCTCA TGGGTGAAGTCACCAATCAGTCCTTCCGCATCACCATCCTTCCTCAGCAATACCTACGGCCGGTGGAGGA CGTGGCCACGTCCCAAGACGACTGTTACAAGTTCGCTGTCTCACAGTCATCCACGGGCACTGTTATGGGA GCCGTCATCATGGAAGGTTTCTATGTCGTCTTCGATCGAGCCCGAAAGCGAATTGGCTTTGCTGTCAGCG CTTGCCATGTGCACGATGAGTTCAGGACGGCGGCAGTGGAAGGTCCGTTTGTTACGGCAGACATGGAAGA CTGTGGCTACAACATTCCCCAGACAGATGAGTCAACACTTATGACCATAGCCTATGTCATGGCGGCCATC TGCGCCCTCTTCATGTTGCCACTCTGCCTCATGGTATGTCAGTGGCGCTGCCTGCGTTGCCTGCGCCACC AGCACGATGACTTTGCTGATGACATCTCCCTGCTCAAGTAAGGAGGCCCGTGGGCAGATGATGGAGACGC CCCTGGACCACATCTGGGTGGTTCCCTTTGGTCACATGAGTTGGAGCTATGGATGGTACCTGTGGCCAGA GCACCTCAGGACCCTCACCAACCTGCCAATGCTTCTGGCGTGACAGAACAGAGAAATCAGGCAAGCTGGA TTACAGGGCTTGCACCTGTAGGACACAGGAGAGGGAAGGAAGCAGCGTTCTGGTGGCAGGAATATCCTTA GACACCACAAACTTGAGTTGGAAATTTTGCTGCTTGAAGCTTCAGCCCTGACCCTCTGCCCAGCATCCTT TAGAGTCTCCAACCTAAAGTATTCTTTATGTCCTTCCAGAAGTACTGGCGTCATACTCAGGCTACCCGGC ATGTGTCCCTGTGGTACCCTGGCAGAGAAAGGGCCAATCTCATTCCCTGCTGGCCAAAGTCAGCAGAAGA AAGTGAAGTTTGCCAGTTGCTTTAGTGATAGGGACTGCAGACTCAAGCCTACACTGGTACAAAGACTGCG TCTTGAGATAAACAAGAACCTATGCGATGCGAATGTTTATACTCCTGGGGGCAGTCAAGATGAGGAGACA GGATAGGATAGAGACAGGAAGGAGATGGTAGCAAAACTGGGAAAGGCAGAACTCTGATCACTTTCTAGTT CCAAGTTTAGACTCATCTCCAAGACAGAAGCCCATCTGGACTAAGAGGTATCATTCCCCAATGTGCCTGT GGTTGTAGTCTGAACTGAAATGAAATGGGGGAAAAAGGGCTTATTAGCCAAAGAGCTCTTTTTAACACTC TTAGAGGAACAGTGCTCATGAGAAAAGTCCCACTGGACAGATGAATTCCTATCTTGTTAATTCTGTCTCT CTCTGCTTCTTCAACATGCTAAGTGGCACCAAAATGACCCAACCCCAAGGTCTTAGGTGCCCTATGGGAC AACAGTTAGAATATTGTAGGGCTAGGGATGGTCTTCCCAGCATAGGTTCACTCCAACCAAGGTGCTAAAA GGAACAGACAGGAGAGTCCTCCTCTCTGATCCACAAAGGCAGAGCCCTCAAGATTCATCCAGCAGGGTTA GGGCTGATGCATTTGCCTCTGCCTGGATTTTGTTTTTATTTTCTTTCTTTTTGCCCAGTGGTACAAAACG ATAAGCTCTTTATGGAATACTGAGTGGGTTCATTCCTCTCTTGCCCTCTCCAATGGCCCCTCTATTTATC TGGCTAAGGAAACACCACGCATTGGCTAGTATTAAACAGCAACTGTAAGATAGAGGGCTTTCTGTTCTAT GTCATTGCCTTCAGTATCAAGGCTGCCTGGAGAAAGGATGGCAGCCTCAGGGCTTCCTTACTTTCTTCTC CTTTCCTGACAGAGCAGCCTTTCTGTCCTGCTCTCTGCTGCCCCTCCCAATATAATCCATGGGTACCCAG GCTGGTTCTTGGGCTAGGTTGTGGGGGCCACACTCACCTCTTCCCTGCCAGTTCTAACACGACAGACATG AAGCCAGTGTTAGTGGGAAGAGCTGGGTTTTCCCAGGATGACCACTGCATCCTCTCCTGGTACGCTCTAC ACTGCTTTCAGGCTGGGGACCTGCCAAGTGTGGGACAGTTGATGAGGAAGAGACATTAGCAGGGCCTCTG GAGTTGCTGGCCCAGCCAGCTGCCCACAAGCCATAAACCAATAAAATAAGAATCCTGCGTCACAGTTTCC AGCTGGGTCCTCTTCCTTGCCCTCGCACTGGTGCTGCTCTGGCTGAGTAGGAATACACCCACAGACTGCC AGGAAGATGGAGACTGTCCGCTTCCGGCTCAGAACTACAGTGTAATTAAGCTTCCAGGATCACTACCATG AAAACGCCGCATTCTGCTTTATCATTTCTACCCATGTTGGGAAAAACTGGCTTTTTCCCCATTTCTTTAC AGGGCAAAAAAAAAAAAAAAAAAAGGGAGAGAGAGAGAGAACTCAACCTAGTTGTTATTTACCCTAGTAA CTGGTGTTCTATTTTTTTTTAAAGGGGGAAAATTTGCATTTATTTTTCTTTTGATGGTTAACTCCTTTGT ATCATAAAATTATGAACTCTGATATGTAAAACAGAAAAAAATCTTGACAACAAAAAAAAAAAAAAAAAA

Restriction Sites

AscI-NotI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction; Metabolism

Relevant Diseases

Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

BACE1

Official Name

BACE1

Full Name

Beta-secretase 1

Alternative Names

ASP2; BACE; HSPC104; beta-secretase 1

Gene ID

23621(Human); 23821(Mouse); 29392(Rat)

Uniprot ID

P56817(Human); P56818(Mouse); P56819(Rat)