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Creative Biolabs

HOT Mouse Hippocampal Neuron Cell HT22

[CAT#: NCL2110P001] Review(5) Q&As(3)

Species:
Mouse
Applications:
Cell Culture
Cell Types:
Neurons

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Product Overview

Description

Mouse hippocampal neuron cells HT22 were immortalized by SV40.

Features

Characteristics: Highly sensitive to glutamate and is therefore used to study glutamate-induced toxicity in neuronal cells.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [tsA58].
Omics: Secretome proteome analysis.

Cell Types

Neurons

Applications

Cell Culture

Species

Mouse
Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM high glucose medium + 10% FBS + 1% P/S

Biosafety Level

1

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
They are easy to culture and maintain
The cells are incredibly robust and have a high rate of viability after thawing. We've used them in several assays, including oxidative stress studies and neurotoxicity tests, and they have consistently provided reliable and reproducible results.
user
Excellent
These cells exhibit typical neuronal properties
The cells' morphology is ideal for our imaging studies, and their neuronal characteristics are well-preserved. We've conducted multiple transfections, and the efficiency has been impressive. Overall, these cells are an excellent model for studying hippocampal neuron function.
user
Excellent
Facilitated our research
These cells are highly responsive to oxidative stressors. We've also had success with various staining techniques and fluorescence microscopy, thanks to the cells' clear neuronal morphology.
user
Excellent
A valuable resource
They are robust and easy to handle, with a consistent growth rate. We've used them in various assays, including immunocytochemistry and Western blotting, and the results have been highly reproducible. Their response to different treatments has provided valuable insights into our research.
user
Excellent
We have been using the HT22 cell line for over two years in our neurobiology lab
The HT22 cell line has been a versatile and reliable tool in our studies on synaptic function. We've used them extensively for electrophysiological recordings, and the quality of the data has been excellent. Their responsiveness to excitotoxic agents has also been particularly useful for our research.
Q&As
What are the recommended passage numbers for HT22 in experiments?
We recommend using mouse hippocampal neuron cell HT22 at early to mid-passage numbers (around 5-15 passages) to ensure optimal neuronal characteristics and reproducibility in experimental outcomes. Regular monitoring and subculturing maintain cell health and functionality.
How long can neuron cell HT22 be maintained in culture without significant changes in phenotype?
Mouse hippocampal neuron cell HT22 can be maintained in culture for extended periods, typically retaining neuronal morphology and function for up to 20 passages under appropriate culture conditions. Regular media changes and careful handling preserve cell integrity and experimental reliability.
Can neuron cell HT22 be genetically modified or transfected for specific research purposes?
Yes, mouse hippocampal neuron cell HT22 is amenable to genetic modification and transfection techniques, allowing researchers to introduce plasmids, siRNAs, or other nucleic acids for studying gene function, protein expression, and signaling pathways relevant to neurobiology.
For Research Use Only. Not For Clinical Use.
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