Mouse Microglia

Positive for F4/80

Species:: Mouse

Cell Types:: Microglia

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Product Overview

Description

Mice microglia (MMcg) are primary mouse microglia derived from CD1 mouse brains on the first day after birth. When resurrected, inoculated and cultured under the recommended conditions, MMcg forms adherents bring up.

Microglia originate from hematopoietic stem cells in the bone marrow and are resident macrophages in the central nervous system (CNS). Due to the presence of the blood-brain barrier, they are separated from the rest of the body. Microglia actively investigate the surrounding area and respond by removing damaged nerve cells, plaques and infectious agents. Because microglia have the function of immune response and maintaining homeostasis in the central nervous system, they are related to neurodevelopment, central nervous system plasticity and repair, neuroinflammation, aging and neurodegeneration, neuropathic pain and infection.

Cell Types

Microglia

Species

Mouse

Properties

Tissue Source

Normal, healthy mouse brain

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Scientific Resources

[Biochemical Methods and Techniques of Neuroscience]

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A Comprehensive Exploration of Microglia Markers

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Well-characterized and consistently provided reliable data for our assays

The mouse microglia provided by this supplier has been instrumental in our research on neuroinflammation. The cells are consistently viable and exhibit the expected morphology, which has significantly advanced our understanding of microglial behavior in neurodegenerative diseases.

Excellent

They have consistently met our research needs

We used these mouse microglia in our recent experiment on synaptic pruning. The cells were easy to culture and maintained their functionality throughout our study, providing reliable data for our research.

Excellent

They were easy to handle, and their behavior in culture was consistent

The quality of the Mouse Microglia was exceptional. They were highly responsive to cytokine stimulation, which was crucial for our assays. The cell lines also showed stable performance across multiple passages.

Excellent

The quality is outstanding

The cells were supplied in excellent condition and facilitated our investigation into the role of microglia in brain injury models.

Excellent

A key component of our research

We were very impressed with the consistency and purity of these Mouse Microglia. They performed well in our inflammation assays, providing clear and reproducible results that have contributed significantly to our project.

Q&As

How long can I culture mouse microglia before they need to be subcultured?

Mouse microglia typically require subculturing every 3 to 4 days, depending on their growth rate and confluency. It is important to monitor the cells regularly under a microscope. Subculture when the cells reach 70-80% confluency to avoid overgrowth and maintain their functionality.

What is the typical passage number of the mouse microglia provided?

The mouse microglia provided are usually at passage 2 to 4. This range ensures that the cells are still in a relatively early stage of growth, which is ideal for maintaining robust functionality and reproducibility in experiments. For best results, we recommend using the cells at this passage range.

How should I handle and store mouse microglia upon arrival?

Upon arrival, mouse microglia should be immediately placed in a 37°C incubator with 5% CO₂. The cells are typically delivered in cryovials containing a cryopreservation medium. Avoid repeated freeze-thaw cycles to maintain cell integrity. If not using immediately, the cryovials can be stored in liquid nitrogen for long-term storage.