Mouse Microglia Cell Line BV-2, Immortalized

Species:: Mouse

Applications:: Cell Culture

Cell Types:: Microglia

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Product Overview

Description

Mouse microglia Bv-2 cells are derived from C57BL/6 mouse microglia. They express nuclear v-myc and chromosome v-raf oncogenes, and express envgp70 antigen on their surface. They have morphological, phenotypic and functional features. Characteristics of phagocytes. This library contains a variety of fluorescently labeled BV2 cells.

Features

Transformant: Recombinant retrovirus J2.
Omics: Secretome proteome analysis.
Omics: Transcriptome analysis.

Cell Types

Microglia

Applications

Cell Culture

Species

Mouse

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM + 10%FBS + 1%GlutaMAX-1 Glutamine + 1% HEPES 1M Buffer solution + 1% P/S

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Publications

Publications (0)

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Scientific Resources

[Biochemical Methods and Techniques of Neuroscience]

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The cells are easy to maintain and have a high transfection efficiency

It's incredibly reliable, with consistent responses to stimuli across experiments. The cell line's stability is impressive, allowing us to perform long-term studies without unexpected variability.

Excellent

A crucial asset for our studies

BV-2 cells have provided consistent and reliable data for our cytokine release assays. The cell line is highly responsive to various stimuli, and we appreciate the stability it offers for extended experiments.

Excellent

Our research has greatly benefited from BV-2 cells

The cell line demonstrates robust activation in response to pro-inflammatory stimuli, making it an ideal model for studying microglial responses. The ease of use and reproducibility are major advantages.

Excellent

The reproducibility of results and ease of culture are particularly noteworthy

We use BV-2 cells to investigate the role of microglia in Alzheimer's disease models. The cell line is very stable and provides consistent results, which is essential for our longitudinal studies. The ease of maintaining these cells has also streamlined our workflow significantly.

Excellent

BV-2 cells have proven to be a reliable model for studying microglial activation

The cell line's consistent behavior in response to various treatments has been crucial for our experiments. We appreciate the detailed protocol provided, which has ensured smooth culturing and handling.

Q&As

How stable are BV-2 cells over multiple passages, and what is the recommended passage range for experiments?

BV-2 cells are relatively stable over multiple passages, but it is recommended to use cells within a passage range of 5-20 for most experiments to minimize variability and maintain cellular functionality. Beyond this range, there may be changes in cell behavior and responsiveness, so it is important to monitor the cells closely and periodically verify their characteristics through functional assays and marker expression.

Can BV-2 cells be genetically modified, and if so, what methods are commonly used?

Yes, BV-2 cells can be genetically modified using various methods such as transfection, lentiviral transduction, or CRISPR/Cas9 gene editing. These techniques allow researchers to overexpress or knockdown specific genes to study their effects on microglial function and behavior. Genetic modification of BV-2 cells enables detailed investigations into the molecular mechanisms underlying microglial responses and the identification of potential therapeutic targets.

What are the advantages of using BV-2 cells for drug screening and testing?

BV-2 cells offer several advantages for drug screening and testing, including their ease of culture, reproducibility, and high-throughput capability. They provide a consistent and reliable model for assessing the effects of potential therapeutic compounds on microglial activation and function. Additionally, BV-2 cells can be used in various assays to evaluate cytotoxicity, anti-inflammatory properties, and neuroprotective effects, making them a valuable tool in the early stages of drug development.