Mouse Neonatal Dorsal Root Ganglion (DRG) Neurons

Features

Terminally differentiated cells

Cell Types

Neurons

Species

Mouse

Size

≥500,000 cryopreserved cells per ampule

Form

Cryopreserved

Culture Medium

Contains B-27 Supplement, Penicillin, Streptomycin, etc.

Shipping

Dry ice

Storage

Liquid Nitrogen Vapor Phase

Handling Advice

Once received the cells: store in liquid nitrogen to keep the cells frozen or thaw cells according to the protocol for culture.
1. Coating T25 flasks:
a. Dissolve 10mg poly-d-lysine, (70-150kd molecular weight), in 100ml sterile water to 0.1mg/ml. Add enough poly-d-lysine solution to cover culture surface. Incubate 1-24 hours. Remove poly-lysine solution and rinse the plate thoroughly. Store coated plates room temperature or 4-8°C.
b. If you are using the coated flask the same day, add about 5 ml of DRG media to the coated flask.
*If the media changes color from pink to yellow, aspirate and discard the media. Add 5ml of fresh DRG media to the coated flask.
2. Thaw the cells in a 37°C water bath. Once you see a small amount of ice left in the vail, spray the vail with 70% Ethanol and wipe it down.
3. Transfer the vail into your Biosafety cabinet.
4. Using a 2 or 5ml pipet, pipet the cells out of the vial. Transfer your cell suspension in to your coated flask (which contains 5 ml media).
5. Close the cap. Make sure cells are evenly distributer in the flask by moving the flask left and right five times. Move it up and down for and additional five times.
6. Place flask in a 37°C incubator with 5% CO2. If flask is not vented, please loosen cap.
7. Change media after 48 hours.
8. Place flask in 37°C incubator until cells are at 90% confluence. Change media every 2-3 days.
Note: Due to the special adherence of primary cells, when the adherent primary cells are transferred to other experimental vessels (such as glass slides, culture plates, confocal culture dishes, etc.) after digestion, the experimental vessels need to be coated to enhance the cells adherence.

Research Use Only

For research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Quality Control

•Test negative for mycoplasma, bacteria, yeast, and fungi
•HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
•A Certificate of Analysis is provided for each cell lot purchased