Neurodegenerative Disease Modeling Services
Neurodegenerative diseases, including Alzheimer's, Parkinson's, and Huntington's disease, are characterized by the progressive degeneration of neurons, leading to cognitive impairment and motor dysfunction. Gaining insights into these intricate conditions is vital for creating successful treatment options and enhancing the quality of life for affected individuals. Disease modeling in neuroscience plays a pivotal role in this endeavor, enabling researchers to dissect the underlying mechanisms and identify potential therapeutic targets.
Creative Biolabs' in vitro modeling approaches utilize advanced iPSC technology and 3D architectures to closely mimic the brain environment. These models not only replicate the cellular and biochemical features of neurodegenerative diseases but also facilitate the evaluation of new drug candidates, ultimately aiding the fight against neurodegenerative diseases.
Fig.1 In vitro brain models for neurodegeneration research.1
Our Neurodegenerative Disease Models
Our in vitro models for neurodegenerative diseases provide a reliable platform for understanding disease mechanisms, facilitating drug discovery, and ultimately advancing therapeutic strategies tailored to combat these complex neurological disorders.
AD In Vitro Modeling | PD In Vitro Modeling |
HD In Vitro Modeling | ALS In Vitro Modeling |
MS In Vitro Modeling | FXS In Vitro Modeling |
Epilepsy In Vitro Modeling | Lesion Induced Cellular Model Development |
2D Cell Models
- Primary cells
Primary cells provide a closer resemblance to the native environment of neurons, as they are directly isolated from tissues. This leads to more physiologically relevant responses and better modeling of disease progression, offering insights into disease mechanisms that are more applicable to human biology.
- Immortalized cell lines
Immortalized cell lines offer consistency and reproducibility, making them an ideal choice for high-throughput screening and mechanistic studies. These cells are easy to maintain and can be genetically modified, allowing for the exploration of specific genetic factors involved in neurodegenerative diseases.
- Human iPSC-derived cells
Human iPSC-derived cells are invaluable because they can be generated from patients, capturing disease-specific phenotypes and genetic backgrounds. This enables the modeling of neurodegenerative diseases with a personalized approach, facilitating the development of potential therapeutic interventions tailored to individual patients.
Precision Modeling of Neurological Disorders: A Guide to Cell Source Selection
Disease | Primary Cell Applications | Immortalized Cell Line Applications | iPSC Applications |
---|---|---|---|
AD | Acute Aβ toxicity testing (primary hippocampal neurons) | High-throughput drug screening (SH-SY5Y overexpressing APP) | Patient-specific Tau pathology (iPSC-derived neurons) |
PD | Electrophysiology of dopaminergic neurons (primary midbrain neurons) | α-synuclein aggregation kinetics (HEK293 model) | LRRK2 mutation mechanisms (iPSC-derived dopaminergic neurons) |
HD | Striatal neuron death analysis (primary cortical-striatal co-culture) | Rapid detection of mHTT aggregation (Neuro2a cells) | CAG repeat dynamics (iPSC-derived striatal neurons) |
Epilepsy | Epileptiform discharge recording (primary hippocampal neuronal networks) | Ion channel drug screening (HT-22 cells) | SCN1A mutation mechanisms (iPSC-derived GABAergic neurons) |
LS | Acute mitochondrial damage (primary neuronal metabolic analysis) | Complex I inhibition model (SH-SY5Y) | SURF1 mutation rescue experiments (iPSC-derived neurons + CRISPR repair) |
MS | Demyelination model (primary oligodendrocyte precursor cells) | Immune cell migration assays (HMC3 microglial cells) | HLA-DRB1 genotype effects (iPSC-derived oligodendrocytes) |
Lesion Induced | Oxygen-glucose deprivation injury (primary neuron-astrocyte co-culture) | Scar factor screening (U87MG astrocyte line) | Ischemic tolerance studies (iPSC-derived endothelial cells + BBB model) |
FXS | Dendritic spine dynamics observation (primary cortical neurons) | Rapid FMRP loss phenotype modeling (HEK293T) | CGG repeat expansion mechanisms (FXS patient iPSC-derived neurons) |
3D Cerebral Organoids
- Unguided brain organoids
These organoids are derived from pluripotent stem cells and develop spontaneously, recapitulating key aspects of early brain development without external guidance.
- Regionalized organoids
By patterning stem cells, regionalized organoids can mimic specific brain regions, enabling the study of region-specific pathologies and functions in neurodegenerative conditions.
- Assembloids
Assembloids are sophisticated structures formed by integrating multiple organoids, allowing for the investigation of inter-regional interactions and their role in neurodegenerative disease mechanisms.
- Bioengineered organoids
These organoids are enhanced using biomaterials and external stimuli, providing a more controlled environment that can emulate complex in vivo conditions to study disease progression and potential therapies.
Microfluidics
- BBB-on-chip
BBB-on-chip provides a functional blood-brain barrier microenvironment that is essential for effective drug testing in neurodegenerative disease research.
- Neuroinflammation Models
The neuroinflammation models effectively simulate residual and peripheral inflammatory responses, allowing for a deeper understanding of neurodegenerative processes.
- Multi-Organ Chip Models
Multi-organ chip models replicate organ crosstalk, including vital gut-brain and lung-brain interactions, enhancing the study of complex neurodegenerative mechanisms.
Bioengineered Tissue Models
- Biomaterials
Bioengineered tissue models utilize advanced biomaterials that promote enhanced neurogenesis and neural differentiation, offer a variable choice of materials, and significantly reduce cell death while facilitating vascularization in brain models.
- Bioprinting
Employ bioprinting technology to achieve precise spatial control, enhancing the integration of multiple cell types and materials, which allows for high reproducibility and scalability in our neurodegenerative disease research.
Related Services
AD In Vitro Assay | PD In Vitro Assay |
ALS In Vitro Assay | HD In Vitro Assay |
Epilepsy In Vitro Assay | MS In Vitro Assay |
Fragile X Syndrome In Vitro Assay |
Reference
- Yan, Yuwei, and Ann-Na Cho. "Human Brain In Vitro Model for Pathogen Infection-Related Neurodegeneration Study." Int J Mol Sci. 2024;25(12):6522. Distributed under Open Access License CC BY 4.0 without modification.
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