NeuroMab™ Anti-Alpha Synuclein Antibody(NRP-0422-P614)
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- DB; ELISA; FC; IHC; In Vitro
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
The antibody has a high affinity for aggregated forms of α-synuclein and a low affinity for monomeric forms of α-synuclein. The antibody is useful in the diagnosis of neurodegenerative diseases.
Species Reactivity
Human
Clonality
Monoclonal
Host Species
Mouse
Applications
DB; ELISA; FC; IHC; In Vitro
Relevant Diseases
Parkinson's Disease
Product Properties
Formulation
PBS only
Preservatives
BSA Free
Concentration
1mg/mL
Purification
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Purity
>95% by SDS PAGE or HPLC
Endotoxin Level
Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg
Low Endotoxin < 1 EU/mg
Shipping
Gel Packs
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Research Use Only
For research use only
Target
Product Pictures
ELISA
Figure 1 shows the results of inhibition ELISA using this antibody.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
DB
Figure 2 shows the Pepscan results of a dot blot epitope assay for the monoclonal antibodies Syn-F1, Syn-F2, Syn-O1, Syn-O2, Syn-O3 and Syn-O4, and the Syn-1 antibody as a control.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
ELISA
Figure 3 shows the results of a sandwich ELISA for the detection of phosphorylated Ser129-α-synuclein aggregates.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
IHC
Figure 4 shows the immunohistochemical analysis of Parkinson's disease tissue.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
FuncS
Figure 5 shows the percentage of dot-positive cells for each antibody tested.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
Publications
Publications (0)
Neural Antibodies
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentAs a postdoc working on synaptic proteins, I found the antibody to be highly reliableIt provides clear and distinct bands in Western blotting and excellent staining in immunofluorescence. The antibody has significantly improved our ability to detect alpha-synuclein in various samples.
ExcellentGave us confidence in our findingsWe use the antibody for detecting alpha-synuclein in both human and mouse samples. The cross-reactivity is minimal, and the results are consistent and reliable. This has been a valuable tool in our study of neurodegenerative disorders.
ExcellentHighly recommended for any neuroscience labIn our quest to understand the pathology of Parkinson's disease, the antibody has been indispensable. The antibody's performance in immunofluorescence microscopy is particularly noteworthy, providing clear and detailed images of alpha-synuclein localization.
ExcellentIt's a top-tier product for alpha-synuclein detectionWe've been using the antibody for over a year now, and it's never disappointed. The antibody provides strong, clear bands in our Western blots and beautiful staining in our immunohistochemistry studies.
ExcellentA reliable reagentWe've been using the antibody for our studies on alpha-synuclein aggregation. The results have been consistently excellent, with clear detection of oligomers and fibrils in our assays. This antibody is a must-have for anyone researching synucleinopathies.
Q&As
Are there any recommended positive controls for this antibody?
We recommend using brain tissue from Parkinson's disease models or other neurodegenerative disease samples as positive controls when using the antibody. These samples typically have elevated levels of alpha-synuclein, providing robust positive signals for your immunodetection assays.
Does this antibody cross-react with beta or gamma-synuclein?
No, the antibody is highly specific to alpha-synuclein and does not significantly cross-react with beta or gamma-synuclein. This specificity is crucial for studies focusing on the role of alpha-synuclein without interference from other synuclein family members.
For Research Use Only. Not For Clinical Use.
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