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Creative Biolabs
Product

NeuroMab™ Anti-Amyloid Beta 1-15 Antibody, Clone 3D6v1

[CAT#: NRP-0422-P643]

A functional antibody raised against Human, Cynomolgus Monkey β-amyloid.

Host Species:
Humanized
Species Reactivity:
Human; Cynomolgus Monkey
Applications:
ELISA; FC; IHC; In Vitro; In Vivo

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Product Overview

Description

The antibody is effective in disappearing Aβ plaques and can be used in or for the treatment, prevention or diagnosis of amyloidogenic diseases.

Species Reactivity

Human; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Humanized

Clone Number

3D6v1

Applications

ELISA; FC; IHC; In Vitro; In Vivo
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

Amyloid Beta 1-15

Official Name

Amyloid Beta

Full Name

Amyloid Beta

Alternative Names

APP; Aβ; Abeta; Amyloid β

Gene ID

351 (Human); 11820 (Mouse)

Uniprot ID

P05067 (Human); P12023 (Mouse)
Product Pictures
ELISA

Figure 1 is a graph depicting the binding of Aβ to chimeric 3D6 (PK1614) compared to mouse 3D6.

The sAPPβ/sAPPα ratio changes over time.

ELISA

Figure 2 is a graph depicting the competition of biotinylated 3D6 with unlabeled 3D6, PK1614 and 10D5 for Aβ binding.

The sAPPβ/sAPPα ratio changes over time.

ELISA

Figure 3 depicts the results of an ELISA measuring the binding of humanized 3D6v1 and chimeric 3D6 to aggregated Aβ.

The sAPPβ/sAPPα ratio changes over time.

IHC

Figure 4 Immunohistochemistry of PDAPP brain sections showing the specificity of the h3D6v1 antibody.

The sAPPβ/sAPPα ratio changes over time.

ELISA

Figure 5 Competitive binding assay of h3D6.

The sAPPβ/sAPPα ratio changes over time.

FuncS

Figure 6 In vitro assay using h3D6v2 antibody. The ability of h3D6v2 to stimulate microglia was tested by an in vitro phagocytosis assay.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.

Publications

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For Research Use Only. Not For Clinical Use.
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