NeuroMab™ Anti-ApoE3 ApoE4 BBB Shuttle Antibody, Clone HJ152

Figure 1 depicts an Figure of a Western blot.

Brain lysates from ApoE KO mice or mice expressing ApoE3, ApoE4, or murine ApoE were immunoblotted with GA-50, HJ151, or HJ152. Western blot showed that HJ151 is specific for ApoE4 and HJ152 recognizes both ApoE3 and ApoE4. GA-50 is a positive control.

Figure 2 depicts graphs showing the body weight of APP/PS1-21 E4/E4 mice injected intraperitoneally with anti-ApoE antibody (1 injection/week for 7 weeks).

Figure 3 depicts a graph showing that HJ151, HJ155, and HJ156 did not significantly reduce APP/PS1-21 E4/E4 mice intraperitoneally injected with anti-ApoE antibody (1 injection/week, a total of 7 times) in PBS fractions of brain tissue homogenates. Soluble Aβ 40 weeks).

Figure 4 depicts unfixed tissue sections (20 μm) from APPPS1-21/apoE4/4 (APPPS1-21/E4), 5XFAD/apoE knockout (5XFAD/EKO) mouse, and human apoE4/4 (human E4) brains. thick) stained with biotinylated (“B”) antibody.

Figure 5 shows antibody detection of ApoE in human plasma lipoprotein particles.

Each shows a graph of the results of various antibody binding assays. Detection of ApoE was observed with HJ153, HJ154, HJ1510, HJ1511, HJ1517, HJ1530, and HJ1534.

Figure 6 shows a schematic representation of the time between 2 and 3.5 months of age with weekly intraperitoneal (IP) injections of PBS, HJ16.6 (IgG negative control) and anti-ApoE antibodies HJ151, HJ155, HJ156.

Figure 7 shows graphs illustrating the number of Aβ plaques in APP/PS1-21 E4/E4 mice treated with weekly intraperitoneal (IP) injections of PBS, HJ16.6 (IgG negative control) and anti-ApoE antibodies HJ151, HJ155 , HJ156 is 2 months old to 3.5 months old.

Figure 8 shows fibrous Aβ plaques measured as percent X-34 stained area in APP/PS1-21 E4/E4 mice injected weekly intraperitoneally (IP) with PBS, HJ16.6 (IgG negative control) Sedimentary chart. , and anti-ApoE antibodies HJ151, HJ155 and HJ156 from 2 to 3.5 months of age.

Figure 9 shows graphs illustrating the number of fibrous Aβ plaques in APP/PS1-21 E4/E4 mice treated with weekly intraperitoneal (IP) injections of PBS, HJ16.6 (IgG negative control) and anti-ApoE antibodies HJ151, HJ155 Figure, and HJ156 from 2 to 3.5 months of age.

Figure 10 is a graph depicting the binding of HJ151 and HJ156 to ApoE4 in amyloid plaques in unfixed mouse brain sections and specificity for heat-induced aggregates of ApoE4.

Unfixed frozen brain sections of APPPS1/APOE4 or APPPS1/APOE KO mice were stained with anti-Aβ antibody HJ3.4, anti-ApoE antibodies HJ151 and HJ156. (Scale bar = 400 µm).

Figure 11 is a graph depicting the binding of HJ151 and HJ156 to ApoE4 in amyloid plaques in unfixed mouse brain sections and specificity for heat-induced ApoE4 aggregates.

Binding of HJ151, HJ153, and HJ156 to untreated recombinant ApoE4 (untreated) and ApoE4 that had been incubated at 40°C for 24 hours (40°C).

Figure 12 depicts the staining of microglia and plaques after acute immunization with HJ156.