NeuroMab™ Anti-FGFR1 BBB Shuttle Antibody(NRZP-1022-ZP3892)

Figure 1 shows ELISA measurement of binding of anti-FGFR1 antibody to purified FGFR ECD fragment.

Figure 2A shows cumulative food intake, blood glucose and body weight changes in lean C57BL/6 mice following a single intraperitoneal injection of 0.5 mpk of R1MAb1 or control IgG.

Figure 2B shows a glucose tolerance test performed with an intraperitoneal injection of 2 g/kg glucose on day 4 after an overnight fast.

Figure 3 shows gene expression analysis using mRNA isolated from mouse liver tissue.

Figure 4 shows gene expression analysis using mRNA isolated from mouse brown adipose tissue.

Figure 5A shows the results of an ELISA measuring antibody binding to biotinylated peptide fragments.

Figure 5B shows the results of an ELISA measuring the binding of His-tagged FGFR1 to FGF2 protein in the presence of various concentrations of R1MAb1 or control IgG. Data are expressed as % FGFR1-His binding and represent mean ± SEM (n=3).

Figure 6 shows non-specific binding in the presence of various concentrations of unlabeled R1MAb1 or FGF21 (reactions also contained BSA (10 mg/ml) and control IgG (350 mM). Data are expressed as total radiolabeled FGF21 in the reaction percent of bound FGF21.

Figure 7 shows the requirement of FGF21 and R1 MAb activity for normal adipose function.

Figure 8 shows blood glucose (left) and body weight (right) of db/db mice after a single intraperitoneal injection of the indicated Abs on day 0 (arrows). N=7 *p<0.05 (control IgG vs. 3 mpk R1MAb 1), **p<0.0005 (control IgG vs. 3 mpk R1MAb1 and control IgG vs. 1 mpk R1MAb 1).

Figure 9 shows Western blot analysis of 3T3-L1 adipocytes treated with 0.5 μg/ml of the indicated proteins for the indicated times.

Figure 10 shows HFD-fed mice performed with GTT in S3B at day 8 after Ab injection. After overnight fasting, mice were intraperitoneally injected with 1 g/kg glucose. Mean body weight was 28.6+/-0.6 (R1MAb1) and 32.1+/-0.8 (control IgG) (p<0.01). N=7.

Figure 11 shows blood glucose (left) and body weight (right) of HFD-fed C57BL/6 mice following a single intraperitoneal injection of R1 Mabl or control IgG at 1 mpk on day 0. N=7～9 *p<0.05.