NeuroMab™ Anti-GARP Antibody(NRP-0422-P1639)
- Host Species:
- Mouse
- Species Reactivity:
- Human; Cynomolgus Monkey
- Applications:
- FC; Inhib; Block; In Vitro; In Vivo
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
The antibody directed against human GARP; inhibits active TGF-β production by human Treg cells. The antibody does not bind mGARP. Its binding to hGARP requires a region comprising amino-acids 101 to 141.
Species Reactivity
Human; Cynomolgus Monkey
Clonality
Monoclonal
Host Species
Mouse
Applications
FC; Inhib; Block; In Vitro; In Vivo
Relevant Diseases
Amyotrophic Lateral Sclerosis
Product Properties
Formulation
PBS only
Preservatives
BSA Free
Concentration
1mg/mL
Purification
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Purity
> 95% (SDS-PAGE)
Endotoxin Level
Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg
Low Endotoxin < 1 EU/mg
Shipping
Gel Packs
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Research Use Only
For research use only
Target
Target
GARP
Official Name
LRRC32
Full Name
Leucine Rich Repeat Containing 32
Alternative Names
Leucine Rich Repeat Containing 32; Glycoprotein A Repetitions Predominant; Transforming Growth Factor Beta Activator LRRC3; Leucine-Rich Repeat-Containing Protein 3; D11S833E; Garpin; GARP; LRRC32
Uniprot ID
Q14392(Human)
Product Pictures
FuncS
Figure 7 MHGARP8 and LHG-10 inhibit the suppressive activity of human Tregs in vitro.
FuncS
Figure 7 MHGARP8 and LHG-10 inhibit the suppressive activity of human Tregs in vitro.
Inhib
Figure 2 MHGARP8 inhibits production of active TGF-β by human Treg clones.
FuncS
Figure 5 MHGARP8 and LHG-10 inhibit human Treg from producing active TGF-β.
FCM
Figure 3 Insufficient presentation of hTGF-β1 on the cell surface for binding by MHGARP8. 293T cells were transfected as indicated below, stained with anti-hLAP antibody or MGARP8, and analyzed by flow cytometry.
FuncS
Figure 6 MHGARP8 and LHG-10 inhibit the suppressive activity of human Tregs in vitro.
Publications
Publications (0)
Neural Antibodies
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentThe antibody's stability and reproducibility are commendableThe antibody's ability to specifically inhibit GARP has been critical for our research on immune regulation. We observed significant changes in our experimental outcomes, which have opened new avenues for exploration.
ExcellentFor blocking experiments in vitroIts effectiveness in flow cytometry has allowed us to accurately analyze cell populations, providing deeper insights into our studies. The antibody's high specificity and affinity for GARP have made it an indispensable tool in our lab.
ExcellentA robust and reliable antibodyThe specificity of the antibody is impressive, and it has significantly contributed to the success of our experiments. We appreciate the consistent performance and the quality of results it provides.
ExcellentWe've had great success with the antibodyThe antibody's effectiveness in blocking GARP has been crucial for our studies on immune modulation. Its specificity and reproducibility have allowed us to generate reliable data.
ExcellentIn our lab, we have been using the antibody for various applicationsThe antibody consistently produces clear and specific staining, with minimal background noise. Its performance in in vitro inhibition studies has also been noteworthy, providing valuable insights into GARP's role in our research focus.
Q&As
We are using this antibody for in Vivo experiment, can you provide the antibody with endotoxin <1 EU/mg?
Yes, we provide customized antibody production and can provide endotoxin-low (<1EU/mg).
What is the recommended concentration for using this antibody in flow cytometry?
For flow cytometry applications, we recommend using the antibody at a concentration of 1-10 µg/mL. However, optimal concentrations may vary depending on the specific experimental conditions and cell types used. It is advisable to perform a titration experiment to determine the best concentration for your particular setup.
Can this antibody be used for in vivo experiments?
Yes, the antibody is suitable for in vivo studies. It has been successfully used in various in vivo models to investigate the function and role of GARP. When planning in vivo experiments, ensure proper administration routes and dosages to achieve the best results while minimizing any potential off-target effects.
How does the antibody perform in blocking assays?
The antibody has demonstrated excellent performance in blocking assays. It effectively inhibits the interaction of GARP with its ligands, allowing researchers to study the functional consequences of this blockade.
For Research Use Only. Not For Clinical Use.
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