NeuroMab™ Anti-LINGO-1 BBB Shuttle Antibody(NRZP-1022-ZP3050)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Applications

WB; IHC; ELISA; FC; In Vitro; In Vivo

Relevant Diseases

Pain

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

Lingo1

Official Name

LINGO1

Full Name

Leucine rich repeat and Immunoglobin-like domain-containing protein 1

Alternative Names

LINGO1; LERN1; LRRN6A; UNQ201; leucine rich repeat and Ig domain containing 1; MRT64

Product Pictures

ELISA

Figure 1 is a graph showing Li113 Fab binding to LINGO-1 measured by ELISA assay.

FuncS

Figure 2 is a graph showing the effectiveness of LINGO-1 monoclonal antibody and Fab in an oligodendrocyte differentiation assay. Bar heights represent MBP concentrations measured by ELISA. Antibodies were tested at concentrations of 1 μg/mL (black), 0.3 μg/mL (dark gray), 0.1 μg/mL (light gray), and 0.03 μg/mL (white).

FuncS

Figure 3 Anti-LINGO-1 antibody promotes myelination in vivo in a lysolecithin assay.

Anti-LINGO-1 antibody promotes in vivo myelination in the lysolecithin assay

ELISA

Figure 4 LINGO-1 ELISA assay showed that Li113 Fab bound to LINGO-1 with an EC50 of 0.09 nM. In the same experiment, the measured EC50 values of Li33 Fab, Li62 Fab and Li81 Fab were 0.30 nM, 0.26 nM and 0.11 nM, respectively.

ELISA

Figure 5 The aggregation state of Li33 Ig2 was further assessed by analytical ultracentrifugation.

FCM

Figure 6 shows that PEGylated Li81 and Li113 can bind to LINGO-1.

ELISA

Figure 7 Determination of the efficacy of Li81 antibody and its fragments by ELISA assay to determine LINGO-1 binding.

ELISA

Figure 8 Determination of the efficacy of Li81 antibody and its fragments by oligodendrocyte proliferation assay ("OPC assay") to determine LINGO-1 binding.