NeuroMab™ Anti-S100A9 Antibody(NRP-0422-P559)

Product Overview

Description

The monoclonal antibody binds to the last 10 amino acids (peptide C7) of the C-terminal region of S100A9.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse; Humanized

Applications

ELISA; WB; Block; In Vitro

Relevant Diseases

Neuroinflammation

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

S100A9

Official Name

S100A9

Full Name

S100 calcium binding protein A9

Alternative Names

S100A9; 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100 calcium binding protein A9

Product Pictures

ELISA

Figure 1 Monoclonal anti-S100A9 antibody binds to the last 10 amino acids (peptide C7) of the C-terminal region of S100A9.

Recombinant human (S100A9) mouse S100A9 (Mu14) (1 μg), PBS1x and C-terminal S100A9 peptide (2 μg) were plated on PVDF membranes and subjected to dot blot analysis using humanized 6B4Hh + Lh or Hc + Lh.

ELISA

Figure 2 shows that mAbs 1B5 and 7A8 bind to the last 10 amino acids of the C-terminal region of S100A9 (peptide C7).

PBS1x, recombinant S100A9, C-terminal and C1 to C7 peptides (50 ng/well) were coated on 96-well plates. Add 100 μl of cell culture supernatant of 11 rabbit blocking anti-S100A9 to the wells. Monoclonal antibody binding was shown using HRP-conjugated goat anti-rabbit IgG antibody. Both mAbs bind to the C7 peptide.

Publications