NeuroMab™ Anti-S100A9 Antibody(NRP-0422-P559)
A functional antibody raised against Human, Mouse S100A9.
- Host Species:
- Mouse; Humanized
- Species Reactivity:
- Human; Mouse
- Applications:
- ELISA; WB; Block; In Vitro
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inquiryDescription
Species Reactivity
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Host Species
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Relevant Diseases
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Concentration
Purification
Purity
Endotoxin Level
Low Endotoxin < 1 EU/mg
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Research Use Only
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Figure 1 Monoclonal anti-S100A9 antibody binds to the last 10 amino acids (peptide C7) of the C-terminal region of S100A9.
Recombinant human (S100A9) mouse S100A9 (Mu14) (1 μg), PBS1x and C-terminal S100A9 peptide (2 μg) were plated on PVDF membranes and subjected to dot blot analysis using humanized 6B4Hh + Lh or Hc + Lh.
Figure 2 shows that mAbs 1B5 and 7A8 bind to the last 10 amino acids of the C-terminal region of S100A9 (peptide C7).
PBS1x, recombinant S100A9, C-terminal and C1 to C7 peptides (50 ng/well) were coated on 96-well plates. Add 100 μl of cell culture supernatant of 11 rabbit blocking anti-S100A9 to the wells. Monoclonal antibody binding was shown using HRP-conjugated goat anti-rabbit IgG antibody. Both mAbs bind to the C7 peptide.
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