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Creative Biolabs

NeuroMab™ Anti-TDP43 BBB Shuttle Antibody(NRZP-1022-ZP2625)

[CAT#: NRZP-1022-ZP2625]

Host Species:
Mouse
Species Reactivity:
Human
Applications:
ELISA; IHC; ICC; Inhib; In Vitro; In Vivo

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Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; IHC; ICC; Inhib; In Vitro; In Vivo

Relevant Diseases

Amyotrophic Lateral Sclerosis
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

TDP43

Official Name

TDP-43

Full Name

TAR DNA binding protein

Alternative Names

TDP-43; TAR DNA binding protein
Product Pictures
DB

Fig.1 depicts that TDP-0 antibody recognizes TDP-43 oligomers specifically.

The 1 ml SEC fractions were collected and subjected to dot blotting by TDP-0 (upper blot) and N -26o antibodies (lower blot).

IHC

Fig.2 depicts that TDP-43 oligomers are present in FTLD-TDP patients.

TDP-0 antibody, in contrast to antibodies for monomeric TDP-43, did not stain nuclei demonstrating specificity toward misfolded TDP-43. Scale bars in all panels are 20 μιη.

ELISA

Fig.3 depicts that TDP-0 mAbs exhibit higher specificities toward TDP-43 oligomer.

Various concentrations of TDP-0 mAbs (1 -2 x 10"^g/mL) respectively produced by TDP-O-3, -5, -8, -9, and -10 hybridoma cells were used in ELISA assay to detect the SDS denatured or non-denatured TDP-43.

ELISA

Fig.4 depicts that TDP-0 mAbs exhibit higher specificities toward purified TDP-43 oligomer.

Conditional medium of TDP-O-3, -5, -8, -9, and -10 hybridoma cell lines were used to detect the TDP-43 oligomers and monomers by ELISA assay.

Cyt

Fig.5 depicts that TDP-0 monoclonal antibody rescues TDP-43 oligomers-induced cytotoxicity.

MTT assay was performed to examine cell viability of BE(2)-C cells. Data are presented as mean ± standard deviation. Statistical analysis was performed by one-way ANOVAs, *p< 0.05, **p< 0.01 , ***p< 0.001 . The result showed the toxicity induced by TDP-43 oligomers was significantly rescued by the treatment of TDP-0 antibody.

IF

Fig.6 depicts that TDP-43 oligomers are present and increase with age in transgenic mouse model of FTLD-TDP.

Immunofluorescent staining of human TDP-43 and TDP-43 oligomers in the brain slices of wild type and 6- and 12-month-old TDP-43 Tg+/+ transgenic mice. Cells with anti-TDP oligomer staining, TDP-43 staining, and DAPI staining are shown (scale bar, 100 μιη). Blocked area was presented at higher magnification in the last column (scale bar, 25 μιη).

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