Mouse Anti-Rat ITGAM Monoclonal Antibody (CBP1589)

Product Overview

Description

Mouse anti-Rat CD11b antibody, clone ED7 recognizes membrane antigens on rat macrophages, monocytes, dendritic cells and granulocytes. Like ED8, ED7 recognizes small branches of microglia in the central nervous system. Except for the cilia of the bronchial epithelium, no other cell types are positive for ED7. The recognized antigens are heterodimers (~160 and ~95 kDa) belonging to the adhesion molecule family (CD11b/CD18). ED7 and ED8 may recognize closely related epitopes on the same molecule. It has been shown that ED7 and ED8 induce homotypic aggregation of granulocytes.

Immunogen

Rat spleen cell homogenate.

Species Reactivity

Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1589

Applications

FC; IHC-Fr

Application Notes

IHC-Fr (1/500)

Relevant Diseases

Alzheimer's Disease

Research Areas

Growth and Development

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

0.5 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD11b

Official Name

ITGAM

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

References

1. Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
2. DeGroot, C.J. et al. (1988) Discrimination between different types of neuroglial cells in rat central nervous system using combined immuno- and enzyme-histochemical methods.
3. Huitinga, I. et al. (1993) Treatment with anti-CR3 antibodies ED7 and ED8 suppresses experimental allergic encephalomyelitis in Lewis rats.

Publications