Mouse Anti-IP-10 Monoclonal Antibody (CBP2142)

Product Overview

Description

Interferon-gamma-inducible 10 kD protein (IP10)is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells, and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP10 to regulate angiogenic activity in cancer and in idiopathic pulmonary fibrosis. IP10 Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified recombinant human protein IP10 (Swiss-Prot P02778).

Immunogen

Purified recombinant human protein IP10 (Swiss-Prot: P02778)

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b,κ

Clone Number

CBP2142

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml.
ELISA: 0.1-1.0 µg/mlRecommended pair for sandwich immunoassay: Capture: A01563, Clone # 1H7D6Detection: A01561, Clone # 1G12E6;A01562, Clone # 2H11E8; A01564, Clone # 1G8E2;Immunohistochemistry: 10-20 µg/ml
Other Applications: User optimized

Conjugation

Unconjugated

Product Properties

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Handling Advice

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CXCL10

Official Name

CXCL10

Full Name

chemokine (C-X-C motif) ligand 10

Alternative Names

gIP-10; INP10; 10 kDa interferon gamma-induced protein; IP-10; C-X-C motif chemokine 10; 1-73; Small-inducible cytokine B10; IFI10; Gamma-IP10; SCYB10; crg-2; C7; mob-1