Mouse Anti-β-Amyloid 1-42 Monoclonal Antibody (CBP2145)

Description

A number of mutations, identified in the gene encoding the β-amyloid precursor protein (βAPP), have been linked to early-onset Familial Alzheimers Disease. βAPP is cleaved sequentially by the proteolytic enzymes β-secretase and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42(43) and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides can bind to scavenger receptors and the receptor for advanced glycation end products. Aβ peptides are degraded either via a reuptake mechanism followed by endosomal degradation or by an extracellular insulin-degrading enzyme. Extracellular accumulation of Aβ leads to formation of aggregates, fibrils, and eventually amyloid deposits called neuritic plaques, a hallmark of Alzheimer's disease. β-amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimers disease.
Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and B-lymphocytes obtained from mouse immunized with a synthetic peptide corresponding to amino acids 36-42 of human β-Amyloid conjugated to KLH (Swiss Prot: P05067).

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse

Clone Number

CBP2145

Applications

IHC; ELISA

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/ml
Dot blot: 0.1-1.0 µg/ml

IHC: 1.0-5.0 µg/ml

Other Applications: user-optimized

Conjugation

Unconjugated

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes: avoid repeated freeze-thaw cycles.

Handling Advice

Reconstitute 40 µg lyophilized powder with 80 µl of deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

APP

Official Name

APP

Full Name

amyloid beta (A4) precursor protein

Alternative Names

CVAP; AAA; AICD-50; PN2; 50; Beta-APP42; AID; Gamma-CTF; S-APP-alpha; 57; AD1; PN-II; Beta-APP40; 42; 40; APPI; Alzheimer disease amyloid protein; Amyloid beta A4 protein; PreA4; ABETA; Amyloid intracellular domain 50; CTFgamma; Amyloid intracellular domain 57; 59; AICD-59; S-APP-beta; APP; AICD-57; Amyloid intracellular domain 59; ABPP; Protease nexin-II; Cerebral vascular amyloid peptide

Gene ID

351(Human);11820(Mouse)

Uniprot ID

P05067(Human);P12023(Mouse)