Mouse Anti-Human Cathepsin A Monoclonal Antibody (CBP2184)

Description

Cathepsin A, also known as Protective Protein Cathepsin A (PPCA), is a serine carboxypeptidase that is required for correct lysosomal transportation. Cathepsin A is synthesized as a 54 kD zymogen and is processed into an enzymatically active two-chain form in lysosomes. Cathepsin A exhibits carboxypeptidase activity at acidic pH in the lysosome and acts such as deaminase and esterase at a neutral pH. It participates in the inactivation of several selected neuropeptides, which includes substance P, oxytocin, and endothelin I. Cathepsin A forms a multienzyme complex with β-galactosidase (GLB1) and neuraminidase (NEU1). It is necessary for the stability and activity for these two enzymes. Genetic defects in the CTSA gene lead to the secondary combined deficiency of GLB1 and NEU1 and cause the lysosomal storage disease galactosialidosis (GS). Cathepsin A has also been suggested to regulate chaperone-mediated autophagy through the proteolytic inactivation of Lysosome-associated membrane protein 2 (LAMP2).

Immunogen

Partial Cathepsin A recombinant protein (29 - 480 a.a.) expressed in 293E cells.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2184

Applications

WB

Application Notes

WB (0.5-2.5 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Cathepsin A

Official Name

CTSA

Full Name

Cathepsin A

Alternative Names

CTSA; GLB2; GSL; NGBE; PPCA; PPGB; cathepsin A

Gene ID

5476 (Human); 19025 (Mouse)

Uniprot ID

P10619 (Human); P16675 (Mouse)

1. Kolli N, et al. 2014. J. Biol. Chem. 189:11592.
2. Takiguchi K, et al. 2000. J. Hum. Genet. 45:200.
3. Hahn CN, et al. 1998. Proc. Natl. Acad. Sci. USA 95:14880.
4. van der Spoel A, et al. 1998. EMBO J. 17:1588.
5. Rudenko G, et al. 1998. Proc. Natl. Acad. Sci. USA 95:621.
6. Galjart NJ, et al. 1991. J. Biol. Chem. 266:14754.