Product
Mouse Anti-Human LC3B Monoclonal Antibody (CBP2252)
[CAT#: NAB2010170LS]
Anti-LC3B Monoclonal Antibody
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- IHC-P; ICC; WB
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
Microtubule-associated protein light chain 3, commonly known as LC3, is a central protein in the autophagy pathway and functions in autophagosome biogenesis. LC3 is a member of the ATG8 protein family and expressed as three isoforms: LC3A (MAP1LC3A), LC3B (MAP1LC3B), and LC3C (MAP1LC3C). Newly synthesized LC3 is hydrolyzed by the cysteine protease ATG4B to produce the active cytosolic form termed LC3I. Through a series of reactions involving other ATGs, LC3I becomes attached to phosphatidylethanolamine. This lipid modified form is termed LC3II, and is involved in autophagosomes membrane expansion and fusion events. LC3 is widely used as a marker for autophagosomes. Defects in autophagy machinery have been associated with multiples diseases including neurodegenerative disorders.
Immunogen
A synthetic peptide corresponding to N-terminal region of human LC3B protein (between amino acid residues 1-50) conjugated to KLH.
Species Reactivity
Human
Clonality
Monoclonal
Host Species
Mouse
Isotype
IgG2b, κ
Clone Number
CBP2252
Applications
IHC-P; ICC; WB
Application Notes
IHC (1.0-5.0 µg/mL); ICC (0.5-1.0 µg/mL)
Relevant Diseases
Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes
Research Areas
Neuroinflammation
Conjugation
Unconjugated
Product Properties
Form
Liquid
Formulation
PBS, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.
Storage
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Handling Advice
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
References
1. Lee J, et al. 2012. Biochem. J. 441(2):523.
2. Martini-Stoica H, et al. 2016. Trends Neurosci. 39(4)221.
2. Martini-Stoica H, et al. 2016. Trends Neurosci. 39(4)221.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
