NeuroMab™ Mouse Anti-TUBB3 Monoclonal Antibody (CBP2724)

Product Overview

Description

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.

Expression Host

Mammalian cells

Immunogen

Beta Tubulin fusion protein Ag0117

Species Reactivity

Human; Mouse; Rat; Nematode; Porcine; Zebrafish

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2724

Applications

IF; IHC; IP; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; IP : 0.5-4.0 ug; WB : 1:500-1:1000

Research Areas

Metabolism

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1891

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1427 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Tubulin β 3

Official Name

TUBB3

Full Name

Beta Tubulin

Alternative Names

Beta tubulin; MC1R; TUBB; Tubulin-beta

Gene ID

10381(Human); 22152(Mouse); 246118(Rat)

Uniprot ID

Q13509(Human); Q9ERD7(Mouse)

References

1. Hei, Z., Wu, S., Liu, Z., Wang, J., & Fang, P. (2019). Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. Journal of Biological Chemistry, 294(13), 4775-4783. 2. Wang, Q., Tian, J., Chen, H., Du, H., & Guo, L. (2019). Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement. Neurobiology of disease, 127, 410-418. 3. Gao, P., Li, N., Ji, K., Wang, Y., Xu, C., Liu, Y.,... & Du, L. (2019). Resveratrol targets TyrRS acetylation to protect against radiation-induced damage. The FASEB Journal, 33(7), 8083-8093.

Publications