NeuroMab™ Mouse Anti-NeuN Monoclonal Antibody (CBP2857)

Product Overview

Description

NeuN is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Expression Host

Mammalian cells

Immunogen

NeuN fusion protein Ag28016

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2857

Applications

IF; IHC; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200

Research Areas

Neuron

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1896

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NeuN

Official Name

NeuN

Full Name

Hexaribonucleotide binding protein 3

Alternative Names

FOX3; Homo sapiens (Human); HRNBP3; NeuN; RBFOX3

Gene ID

146713(Human); 52897(Mouse); 287847(Rat)

Uniprot ID

A6NFN3(Human); Q8BIF2(Mouse)

References

1. Xu, L., Xing, Q., Huang, T., Zhou, J., Liu, T., Cui, Y.,... & Yang, G. L. (2019). HDAC1 silence promotes neuroprotective effects of human umbilical cord-derived mesenchymal stem cells in a mouse model of traumatic brain injury via PI3K/AKT pathway. Frontiers in Cellular Neuroscience, 12, 498. 2. Chen, S., Sun, M., Zhao, X., Yang, Z., Liu, W., Cao, J.,... & Wen, A. (2019). Neuroprotection of hydroxysafflor yellow A in experimental cerebral ischemia/reperfusion injury via metabolic inhibition of phenylalanine and mitochondrial biogenesis. Molecular medicine reports, 19(4), 3009-3020. 3. Vazquez-Villaseñor, I., Garwood, C. J., Heath, P. R., Simpson, J. E., Ince, P. G., & Wharton, S. B. (2020). Expression of p16 and p21 in the frontal association cortex of ALS/MND brains suggests neuronal cell cycle dysregulation and astrocyte senescence in early stages of the disease. Neuropathology and Applied Neurobiology, 46(2), 171-185.

Publications