Anti-Neurofilament L Monoclonal Antibody (CBP3648)

Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are the main component of neuron cytoskeleton, mainly used to provide structural support for axons and adjust the diameter of axons. There are three main NF subunits, and the names of these subunits are based on the apparent molecular weight of mammalian subunits on SDS-PAGE. The light or minimum NF (NF-L) operating speed is 68-70 kD. The operating frequency of medium or medium NF (NF-M) is about 145-160 kD, and the operating frequency of heavy or highest NF (NF-H) is about 200-220 kD. However, due to the highly charged C-terminal region of the molecule, the actual molecular weight of these proteins is significantly lower. The level of NF gene expression is related to the axon diameter, which controls the speed of electrical signals propagating along the axon. Mutant mice with NF abnormalities have a phenotype similar to amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful to distinguish between glial cells (positive for NF) and glial cells (negative for NF).

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP3648

Applications

IHC-P

Application Notes

IHC-P (5.0-10 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Alexa Fluor 594; Alexa Fluor 488; Alexa Fluor 498; Alexa Fluor 647; Alexa Fluor 700; APC; APC/Cyanine 7; APC/Fire 750; Biotin; Brilliant Violet 421; Brilliant Violet 510; Brilliant Violet 570; Brilliant Violet 570; Brilliant Violet 605; Brilliant Violet 650; Brilliant Violet 711; Brilliant Violet 750; Brilliant Violet 785; FITC; HRP; PE; Pacific Blue; PE/Cyanine 5; PE/Cyanine 7; PerCP; Spark YG 570

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Neurofilament L

Official Name

NEFL

Full Name

Neurofilament light polypeptide

Alternative Names

NEFL; CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110; neurofilament; light polypeptide; neurofilament light; CMTDIG

Gene ID

4747 (Human); 18039 (Mouse); 83613 (Rat)

Uniprot ID

P07196 (Human); P08551 (Mouse); P19527 (Rat)

1. Zetterberg H, Blennow K. 2015. JAMA Neurol. 11:1237-8.
2. Bäckström D, et al. 2015. JAMA Neurol. 72(10):1175.
3. Janelidze S, et al. 2015. Neurology 85:1834.
4. Magdalinou NK, et al. 2015. J. Neurol. Neurosurg. Psychiatry 86(11):1240.
5. Skillbäck T, et al. 2014. Neurology. 83:1945.