Mouse Anti-Lamin B1 Monoclonal Antibody (CBP4366)

Description

Lamin B1 is a type V intermediate filament and an integral component of the nuclear lamina, a meshwork of proteins located inside the nuclear membrane. There are 3 major types of nuclear lamin proteins: lamin A/C, lamin B1 and lamin B2. Lamin A and C are the alternative splicing products of LMNA gene, whereas lamin B1 and lamin B2 are encoded by genes LMNB1 and LMNB2, respectively. Lamins are crucial for maintaining the nuclear structure. They are also important for regulating nuclear functions and activities, including DNA replication and transcription, nuclear and chromatin organization, cell cycle regulation, cell development and differentiation, nuclear migration, and apoptosis. In the brain, lamin B1 levels modulate brain development. Loss of lamin B1 in mouse adversely impairs embryonic brain development and corticogenesis. In addition, duplications of LMNB1 gene causes autosome-dominant leukodystrophy, an adult-onset demyelinating disorder characterized by symmetrical widespread myelin loss in the central nervous system with a phenotype similar to chronic progressive multiple sclerosis.

Immunogen

Recombinant human Lamin B1 protein.

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b, κ

Clone Number

CBP4366

Applications

WB; IHC-P

Application Notes

WB ( 0.5-10 µg/mL); IHC-P (1.0-10 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Lamin B1

Official Name

LMNB1

Full Name

Lamin B1

Alternative Names

LMNB1; ADLD; LMN; LMN2; LMNB; lamin B1

Gene ID

Uniprot ID

1. Padiath QS. et al. 2006. Nat Genet. 38(10):1114-23.
2. Camps J. et al. 2015. Nucleus. 6(1):8-14.
3. Mahajani S. et al. 2017. Sci Rep. 7(1):4897.