Mouse Anti-Histone H3 Trimethyl (CBP4376)

Product Overview

Description

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.

Immunogen

This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9.

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP4376

Applications

WB; IHC

Application Notes

WB (1:100-1:1000); IHC (1:50-1:100)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

1 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Histone H3 (C-terminus)

Official Name

H3F3

Full Name

Histone H3

Alternative Names

H3-3A; H3.3A; H3F3; H3 histone; family 3A; H3 histone family member 3A; H3.3 histone A; H3F3A; H3-3B; H3-3B; H3.3B; H3 histone; family 3B (H3.3B); H3 histone family member 3B; H3.3 histone B; H3F3B; H3-3A

Publications