Mouse Anti-Human Histone H3 Monomethyl (CBP4386)

Description

Chromosomal DNA wraps around histone proteins located in the nucleus of the cell. Histone H3 is one of the five main histone proteins. Featuring a main globular domain and a long N-terminal tail, H3 forms nucleosomes with the 'beads on a string' structure. Histone proteins are highly post-translationally modified and Histone H3 is the most extensively modified. The term "Histone H3" does not distinguish between sequence variants or modification state - including three sequence variants H3.1, H3.2, and H3.3. Histone H3 is important in the field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Histone H3 lysine 9 (H3K9) is a major site of chromatin regulation. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids as well as phosphorylation events. Di- and Tri-methylation of Lysine 9 are associated with repression and heterochromatin, while mono-methylation of K4 is associated with active genes and has been associated with gene silencing and the formation of relatively inactive regions of DNA known as heterochromatin. When H3K9 is acetylated, its neutral charge cannot bind to DNA, resulting in greater access of transcriptional enzymes to DNA. When H3K9 is unmodified, less transcription occurs. The greater the methylation on H3K9, the greater the transcriptional repression.

Immunogen

This monoclonal antibody was raised against a synthetic peptide from human histone H3, mono-methylated at Lysine-9.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP4386

Applications

WB; IHC

Application Notes

WB (1:100); IHC (1:1000)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

1 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Histone H3 (C-terminus)

Official Name

H3F3

Full Name

Histone H3

Alternative Names

H3-3A; H3.3A; H3F3; H3 histone; family 3A; H3 histone family member 3A; H3.3 histone A; H3F3A; H3-3B; H3-3B; H3.3B; H3 histone; family 3B (H3.3B); H3 histone family member 3B; H3.3 histone B; H3F3B; H3-3A