- NeuroMab™ Anti-EPHB2 Antibody,Clone NR1370P (Cat#: NRP-0422-P1220)
- NeuroMab™ Anti-Alpha Synuclein BBB Shuttle Antibody,Clone NR1707P (Cat#: NRZP-1022-ZP4050)
- Mouse Anti-Human α-Synuclein Phospho (Tyr39) (CBP3706) (Cat#: NAB201250LS)
- NeuroMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- NeuroMab™ Anti-F-Spondin/SPON1 Antibody, Clone N24875P (CBP11839) (Cat#: NRZP-0822-ZP4740)
- NeuroMab™ Anti-Tau Antibody,Clone NR2946P (Cat#: NRP-0422-P1686)
- NeuroMab™ Anti-Amyloid Beta 1-15 Antibody,Clone NR2660P (Cat#: NRP-0422-P867)
- NeuroMab™ Anti-GD2 Antibody,Clone NR3007P (Cat#: NRZP-1222-ZP767)
- NeuroMab™ Anti-Integrin αvβ8 BBB Shuttle Antibody,Clone NR2431P (Cat#: NRZP-1222-ZP1218)
- NeuroMab™ Anti-FGFR1 Antibody,Clone NR3547P (Cat#: NRP-0422-P1244)
- Human Hippocampal Neuron Cells HPPNCs (Cat#: NCL2110P106)
- Mouse Retinal Ganglion Cells (Cat#: NCL2110P145)
- iNeu™ Human Schwann Cell (Cat#: NCL-2103-P63)
- iNeu™ Human Sensory Neurons (Cat#: NCL-2103-P62)
- Human Glioblastoma Cell Line SF126 (Cat#: NCL-2108P35)
- iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs) (Cat#: NCL-2103-P49)
- iNeu™ Human Neural Stem Cell Line (Cat#: NCL200552ZP)
- Mouse Glioma Cell Line GL-261-Luc (Cat#: NCL-2108P06)
- Rat Schwann Cells RSC96, Immortalized (Cat#: NCL-2108P21)
- Human Neurons Isolated from Cortex (Cat#: NCL-21P6-023)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- pAAV-hSyn-DIO-XCaMP-R-WPRE (Cat#: NTA-2012AD-P508)
- PRV-CAG-EGFP (Cat#: NTA-2011-ZP14)
- AAV-mDLX-CRE-tdTomato (Cat#: NRZP-0622-ZP721)
- pAAV-syn-jGCaMP8m-WPRE (Cat#: NTA-2106-P062)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- pAAV-EF1a-DIO-EGFP-WPRE (Cat#: NTA-2012AD-P285)
- pAAV-syn-jGCaMP8s-WPRE (Cat#: NTA-2106-P063)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- AAV2/9-hEF1a-DIO-mCherry-P2A-TetTox-WPRE-pA (Cat#: NTA-2012-ZP268)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroPro™ Anti-ASA BBB Shuttle Protein, HIRMab-ASA (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, HIRMab-TNFR (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein, HIRMab-PON1 (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein, 8D3-IL-1RA (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, HIRMab-IDUA (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, HIRMab-GDNF (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-IDS BBB Shuttle Protein, HIRMab-IDS (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-EPO BBB Shuttle Protein, HIRMab-EPO (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, cTfRMAb-GDNF (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein, HIRMab-NAGLU (Cat#: NRZP-0423-ZP506)
New Fluorescent Probes for Detection of Neurotransmitters: GRAB Probes
GRAB Neurotransmitter Fluorescent Probes
Neuroscience research focuses on understanding how neurons connect and communicate with each other at the cellular and molecular levels, which has important implications for analyzing the neural circuits underlying animal cognition and behavior. Manipulating and detecting communication between neurons is a major tool in neuroscience research, and a number of genetically encoded fluorescent probes for the detection of neurotransmitters have been developed by coupling GPCRs and the circulating rearranged fluorescent protein cpFP. These genetically encoded fluorescent probes, named GRAB (GPCR Activation-Based) probes, were used to detect neurotransmitter dynamics in vivo with high spatial and temporal resolution.
Principles of GRAB neurotransmitter Fluorescent Probes
The principle of the GRAB probe lies in the combination of neurotransmitter and GPCR causes the conformational change of the latter, which in turn causes the conformational change of cpFP, which in turn affects the microenvironment around its chromophore, and finally causes its fluorescence intensity to change, which can be detected by fluorescence microscopy.
Fig. 1 The principle of the green neurotransmitter probe (cpEGFP-based).
Fig. 2 The principle of the red neurotransmitter probe (cpmApple-based).
Advantages of Our GRAB Neurotransmitter Fluorescent Probes
- High sensitivity.
- High specificity.
- High temporal and spatial resolution.
- High signal-to-noise ratio.
- Non-invasive.
- No additional test equipment is required.
Creative Biolabs provides a complete list of viral products of these probes (mainly AAV products) to help neuroscientists detect the dynamic changes of transmitters in vivo.
Probe name | Probe type |
ACh | Acetylcholine probe |
DA | Dopamine probe |
NE | Norepinephrine probe |
5-HT | Serotonin probe |
HA | Histamine probe |
Ado | Adenosine probe |
ATP | Adenosine triphosphate probe |
VIP | Vasoactive intestinal peptide probe |
CCK | Cholecystokinin probe |
NPY | Neuropeptide Y probe |
CRF | Corticotropin releasing factor probe |
AVP | Arginine vasopressin probe |
NTS | Neurohypotensive peptides |
eCB | Endocannabinoids |
AEA | Aannabinoids |
If you need a probe not shown above, please directly contact us.
Appendix
Cat | Product Name |
NRZP-0722-ZP130 | pAAV-hSyn-GRAB_ACh3.0 |
NRZP-0722-ZP131 | pAAV-hSyn-GRAB_NE1m |
NRZP-0722-ZP132 | pAAV-hSyn-GRAB_NE3m |
NRZP-0722-ZP133 | pAAV-hsyn-GRAB_DA2m |
NRZP-0722-ZP134 | pAAV-hsyn-GRAB_5-HT1.0 |
NRZP-0722-ZP135 | pAAV-hSyn-GRAB_NE1h |
NRZP-0722-ZP136 | pAAV-hSyn-GRAB_NE1h (AAV Retrograde) |
NRZP-0722-ZP138 | pAAV-hSyn-GRAB_DA1h |
NRZP-0722-ZP139 | pAAV-hSyn-GRAB_DA1h (AAV Retrograde) |
NRZP-0722-ZP140 | pAAV-hSyn-GRAB_DA1m |
NRZP-0722-ZP141 | pAAV-hSyn-DIO-GRAB_ACh3.0 |
NRZP-0722-ZP142 | pAAV-hsyn-GRAB_DA2h |
NRZP-0722-ZP143 | pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX |
NRZP-0722-ZP144 | pAAV-hsyn-GRAB_rDA1m |
NRZP-0722-ZP145 | pAAV-hsyn-GRAB_Ado1.0 |
NRZP-0722-ZP147 | pAAV-hSyn-GRAB_NEmut |
NRZP-0722-ZP148 | pAAV-hSyn-GRAB_NEmut (AAV Retrograde) |
NRZP-0722-ZP157 | pAAV-hsyn-GRAB_DA-mut |
NRZP-0722-ZP158 | pAAV-hsyn-GRAB_rDA1h |
NRZP-0722-ZP159 | pAAV-hsyn-GRAB_rDA-mut |
NRZP-0722-ZP160 | pAAV-hsyn-GRAB_Ado1.0med |
NRZP-0722-ZP161 | pAAV-hsyn-GRAB_Ado1.0mut |
NRZP-0722-ZP162 | pAAV-GfaABC1D-GRAB_Ado1.0 |
NRZP-0722-ZP163 | pAAV-GfaABC1D-GRAB_Ado1.0med |
NRZP-0722-ZP164 | pAAV-GfaABC1D-GRAB_Ado1.0mut |
NRZP-0722-ZP165 | pAAV-EFS-DIO-GRAB_Ado1.0 |
NRZP-0722-ZP166 | pAAV-EFS-DIO-GRAB_Ado1.0med |
NRZP-0722-ZP167 | pAAV-hSyn-GRAB_eCB2.0 |
NRZP-0722-ZP168 | pAAV-hSyn-GRAB_eCBmut |
NRZP-0722-ZP169 | pAAV-hSyn-DIO-GRAB_eCB2.0 |
NRZP-0722-ZP170 | pAAV-hSyn-DIO-GRAB_eCB2.0mut |
NRZP-0722-ZP171 | pAAV-CaMKII-GRAB_eCB2.0 |
NRZP-0722-ZP172 | pAAV-GfaABC1D-GRAB_eCB2.0 |
NRZP-0722-ZP173 | pAAV-EFS-fDIO-GRAB_eCB2.0 |
NRZP-0722-ZP174 | pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX |
NRZP-0722-ZP175 | pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX |
NRZP-0722-ZP177 | pAAV-hsyn-GRAB_ATP1.0 |
NRZP-0722-ZP178 | pAAV-hsyn-GRAB_ATP1.0mut |
NRZP-0722-ZP179 | pAAV-GfaABC1D-GRAB_ATP1.0 |
NRZP-0722-ZP180 | pAAV-GfaABC1D-GRAB_ATP1.0mut |
NRZP-0722-ZP181 | pAAV-EFS-DIO-GRAB_ATP1.0 |
NRZP-0722-ZP182 | pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX |
NRZP-0722-ZP184 | pDisplay-GRAB_HTR6-1.0 |
NRZP-0722-ZP185 | pAAV-hSyn-GRAB_NTS1.0 |
NRZP-0722-ZP186 | pDisplay-GRAB_OT1.0 |
NRZP-0722-ZP187 | pDisplay-GRAB_OTmut |
NRZP-0722-ZP188 | pAAV-EFS-DIO-GRAB_OT1.0 |
NRZP-0722-ZP189 | pAAV-EFS-DIO-GRAB_OTmut |
NRZP-0722-ZP194 | GRAB-SST1.0 Peptide Sensor |
NRZP-0722-ZP195 | GRAB-CCK1.0 Peptide Sensor |
NRZP-0722-ZP196 | GRAB-NTS1.0 Peptide Sensor |
NRZP-0722-ZP197 | GRAB-OX1.0 Peptide Sensor |
NRZP-0722-ZP198 | GRAB-NPY1.0 Peptide Sensor |
NRZP-0722-ZP199 | GRAB-SP1.0 Peptide Sensor |
NRZP-0722-ZP200 | GRAB-GHRL1.0 Peptide Sensor |
NRZP-0722-ZP201 | GRAB-CRF1.0 Peptide Sensor |
NRZP-0722-ZP202 | GRAB-UCN1.0 Peptide Sensor |
NRZP-0722-ZP203 | GRAB-VIP1.0 Peptide Sensor |
NRZP-0722-ZP204 | GRAB-PTH1.0 Peptide Sensor |