Organotypic Brain Slice Assay

Importance of Organotypic Brain Slice Assay

All the central nervous system (CNS) cell types are playing important roles in regulatory functions of the brain, such as maintaining immune homeostasis by phagocytosis, microglia chemotaxis, synaptic transmission, etc. Dysregulation of synaptic transmission leads to numerous CNS disorders, such as Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and psychiatric disorders. The organotypic brain slice mimics a three-dimensional model of the brain that is physiologically similar to the human brain, which can be used to better understand the pathogenesis of all cell types in the CNS which leads to neural inflammation and neurodegenerative disorders. Thus, in the field of neuroscience research, an organotypic slice of the brain has been a useful tool for mechanism and pathogenesis exploration. Organotypic brain slice assays serve as bridging technologies between cell culture and in vivo experiments for the development and evaluation of novel therapeutic targets and strategies. Creative Biolabs offers multiple customized organotypic brain slice assays and relative support to assist our customers in the exploration of mechanisms and therapeutics of neurological diseases.

Organotypic Brain Slice Assay Provided by Creative Biolabs

With the organotypic brain slice, the complete three-dimensional structure of the brain and the cell-cell interactions of the brain can be maintained, which are able to recapitulate the physiological features of the brain. Our organotypic brain slice assay enables the overtime investigation of the brain inflammatory condition, by detecting the release of pro-inflammatory cytokines, such as TNF-alpha, IL-6, and IL-10. Brain slice images are obtained by high-quality fluorescence microscopy with immunohistochemical technology. In addition, gene expression studies with qPCR or NGS techniques can also be used to detect the alteration of organotypic brain slices. Therefore, the organotypic brain slice assay is used to study the functional responses of brain slices with different drug candidates. Furthermore, the emerging technique of electrophysiology also could record stimulus in cultured and acutely dissociated brain slices with multi-electrode arrays (MEAs), which is a great opportunity to study the pathological basis of synaptic plasticity. Brain slice electrophysiology technology with MEA biochips is an ideal approach to record and collect data from an individual neuron to a large number of brain tissues in order to study neurological physiology and related diseases.

Creative Biolabs offers our clients advanced in vitro organotypic brain slice assay, which allows researchers to intuitively study the mechanisms of action within the brain and evaluate the effect of target compounds within an intact neural system through our electrophysiology tools. Researchers could therefore investigate the mechanisms of CNS diseases, the target of interest, and the pharmacological effects of compounds on synaptic transmission or inflammation in brain slices or other specific brain regions. In addition, we provide in vivo histology and ADME pharmacokinetic services to further explore the efficacy and safety of screening compounds, which guarantee you a tailored neurological research and development program. For further information, please don't hesitate to contact us.