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Creative Biolabs
Product

pAAV- EF1a-DI0-GCaMP6f-P2A-nls-dTomato

[CAT#: NTA-2011-ZP70]

Viral vector tools for calcium imaging.

Tracer Type:
Calcium Sensor
Applications:
Calcium Sensor

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Product Overview

Description

Calcium imaging refers to the use of calcium ion indicators to monitor the concentration of calcium ions in cells, tissues or culture media. This technique is widely used to study calcium signaling in neuronal activity in vivo and in vitro.

Creative Biolabs provides a comprehensive list of neural AAV viral tracing tools for calcium imaging.

Tracer Type

Calcium Sensor

Functional Gene

GCaMP6f

Virus Type

Adeno-Associated Virus (AAV)

Applications

Calcium Sensor

Sub Type

AAV packing

Application Notes

How to use calcium virus imaging tools for research?
Step1: Choose the appropriate calcium ion indicator
Calcium ion indicators are divided into chemiluminescent indicators and GECI indicators. Among them, GECI indicators GCaMP6f and GCaMP6s are widely used in in vivo calcium imaging research.
Step2: Transport the calcium ion indicator to the target site
The chemiluminescent indicator is usually pierced by an electrode or entered into the target site by means of acetoxymethyl and dextrose, while the GECI indicator is mainly introduced to the target site by means of viral infection.
Step3: Collect signals and record the results
Calcium imaging technology is usually used in conjunction with microscopy to record changes in calcium ions

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde/Retrograde

Cell Types

Broad Spectrum

Promoter

Ef1α

Key Components

DIO-GCaMP6f

Expression

Cre-dependent
Properites

Fluorophore

dTomato

Research Use Only

For research use only.
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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