pAAV- EF1a-DI0-GCaMP6f-P2A-nls-dTomato
[CAT#: NTA-2011-ZP70]
Viral vector tools for calcium imaging.
- Tracer Type:
- Calcium Sensor
- Applications:
- Calcium Sensor
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SPECIFIC INQUIRY
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Description
Calcium imaging refers to the use of calcium ion indicators to monitor the concentration of calcium ions in cells, tissues or culture media. This technique is widely used to study calcium signaling in neuronal activity in vivo and in vitro.
Creative Biolabs provides a comprehensive list of neural AAV viral tracing tools for calcium imaging.
Creative Biolabs provides a comprehensive list of neural AAV viral tracing tools for calcium imaging.
Tracer Type
Calcium Sensor
Functional Gene
GCaMP6f
Virus Type
Adeno-Associated Virus (AAV)
Applications
Calcium Sensor
Sub Type
AAV packing
Application Notes
How to use calcium virus imaging tools for research?
Step1: Choose the appropriate calcium ion indicator
Calcium ion indicators are divided into chemiluminescent indicators and GECI indicators. Among them, GECI indicators GCaMP6f and GCaMP6s are widely used in in vivo calcium imaging research.
Step2: Transport the calcium ion indicator to the target site
The chemiluminescent indicator is usually pierced by an electrode or entered into the target site by means of acetoxymethyl and dextrose, while the GECI indicator is mainly introduced to the target site by means of viral infection.
Step3: Collect signals and record the results
Calcium imaging technology is usually used in conjunction with microscopy to record changes in calcium ions
Step1: Choose the appropriate calcium ion indicator
Calcium ion indicators are divided into chemiluminescent indicators and GECI indicators. Among them, GECI indicators GCaMP6f and GCaMP6s are widely used in in vivo calcium imaging research.
Step2: Transport the calcium ion indicator to the target site
The chemiluminescent indicator is usually pierced by an electrode or entered into the target site by means of acetoxymethyl and dextrose, while the GECI indicator is mainly introduced to the target site by means of viral infection.
Step3: Collect signals and record the results
Calcium imaging technology is usually used in conjunction with microscopy to record changes in calcium ions
Research Areas
Neural Circuit Mapping
Direction of Transport
Anterograde/Retrograde
Cell Types
Broad Spectrum
Promoter
Ef1α
Key Components
DIO-GCaMP6f
Expression
Cre-dependent
Properites
Fluorophore
dTomato
Research Use Only
For research use only.
Publications
Publications (0)
Neural Tracers
For Research Use Only. Not For Clinical Use.
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