pAAV-hSyn-DIO-XCaMP-R-WPRE

Product Overview

Tracer Type

Calcium Sensor

Functional Gene

XCaMP-R

Virus Type

Adeno-Associated Virus (AAV)

Applications

Calcium Sensor

Sub Type

AAV packing

Research Areas

Neural Circuit Mapping

Promoter

Syn

Expression

Cre-dependent

Properites

Shipping

Viral particles are shipped frozen on dry ice.

Handling Advice

The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-259 handling depending on the insert.

Storage

Store at -80°C. Thaw just before use and keep on ice.

Quality Control

Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.

Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.

Research Use Only

For research use only.

Publications

Publications (0)

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Scientific Resources

[Flyer]

Ready-to-Use AAV Biosensors

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[Knowledge Sharing]

Applications of Calcium Imaging Technology in Nervous System Research

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

A highly reliable product that has exceeded my expectations

I am thoroughly impressed with its performance. The sensor is highly sensitive and provides accurate readings, which has significantly enhanced the quality of my calcium imaging experiments. The packaging was also secure, ensuring that the product arrived in perfect condition.

Excellent

I highly recommend this product to anyone working in the field of calcium imaging

It is easy to use and integrates seamlessly with our existing equipment. The fluorescence signals are robust, and the data is reproducible across different experiments.

Excellent

I have had great success using the calcium sensor in my lab

The sensor is extremely sensitive and provides clear and consistent signals. The user manual is detailed and easy to follow, making the setup process straightforward. This product has undoubtedly improved the accuracy of my calcium measurements.

Excellent

It is one of the best calcium sensors I have used

Its high sensitivity and specificity make it ideal for my neurobiology research. The results are reliable and consistent, which is crucial for my experiments. Additionally, the customer service team was very helpful and responsive to my queries.

Excellent

A positive experience

The sensor is highly sensitive and produces strong fluorescence signals. It has helped me achieve more precise and reliable data in my calcium imaging studies. The product is well worth the investment.

Q&As

What types of experimental conditions or modifications might affect the performance of the pAAV-hSyn-DIO-XCaMP-R-WPRE sensor?

Factors such as the choice of animal model, expression levels of the sensor, and the imaging conditions can impact the performance of the pAAV-hSyn-DIO-XCaMP-R-WPRE sensor. High expression levels might lead to sensor saturation or altered kinetics, while suboptimal imaging conditions can affect signal clarity. Additionally, the physiological state of the neurons and any genetic or pharmacological modifications made to the system should be considered, as they can influence calcium signaling and sensor performance.

What are the recommended methods for delivering the pAAV-hSyn-DIO-XCaMP-R-WPRE sensor into neurons?

The pAAV-hSyn-DIO-XCaMP-R-WPRE sensor is typically delivered into neurons using viral vectors, specifically AAVs. These viruses can be injected into targeted brain regions or administered via stereotaxic surgery. The choice of delivery method depends on the specific experimental goals and the targeted neuronal population. Proper surgical techniques and viral titers are critical for achieving effective and specific expression of the sensor.

What are the expected fluorescence characteristics of the pAAV-hSyn-DIO-XCaMP-R-WPRE sensor, and how should they be interpreted?

The sensor is expected to exhibit fluorescence that varies with intracellular calcium levels. The fluorescence intensity increases upon calcium binding and decreases when calcium levels drop. Researchers should calibrate their imaging systems to accurately measure these changes and interpret the data in the context of the experimental conditions. Comparing fluorescence changes with known calcium concentrations or controls can help in quantifying the calcium dynamics accurately.