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Creative Biolabs

pAAV-hSyn-dLight1.2

[CAT#: NTA-2012AD-P167]

Tracer Type:
Neurotransmitter sensor
Applications:
Dopamine Sensor

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Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

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AAV Serotype:
Size:
Titer:
Product Overview

Tracer Type

Neurotransmitter sensor

Functional Gene

dLight1.2

Serotype

AAV5

Virus Type

Adeno-Associated Virus (AAV)

Applications

Dopamine Sensor

Sub Type

AAV1; AAV5

Research Areas

Neural Circuit Mapping

Promoter

Syn

Key Components

pAAV-hSyn

Expression

Normal Expression
Properites

Titer

≥ 4×10¹² vg/mL

Shipping

Viral particles are shipped frozen on dry ice.

Handling Advice

The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-168 handling depending on the insert.

Storage

Store at -80°C. Thaw just before use and keep on ice.

Quality Control

Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.

Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.

Research Use Only

For research use only.
Gene/Insert

Gene/Insert Name

dLight1.2

Insert Size (bp)

2043

Tag / Fusion Protein

Flag tag (N terminal on insert)
Growth in Bacteria

Bacterial Resistance(s)

Ampicillin

Growth Temperature

37°C

Growth Strain(s)

NEB Stable

Copy number

High Copy
Cloning Information

Cloning method

Restriction Enzyme

5′ cloning site

BaMHI (not destroyed)

3′ cloning site

HindIII (not destroyed)

5′ sequencing primer

ccggccaccttggtcgcgtcc

3′ sequencing primer

ggagaaaatgaaagccatacgggaagc
References

References

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 10.1126/science.aat4422. Epub 2018 May 31.
Publications

Publications (0)

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