pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet
[CAT#: NTA-2012AD-P62]
Intersectional viral expression of ChRmine-oScarlet in cells expressing Cre AND NOT Flp
- Tracer Type:
- Optogenetics
- Applications:
- Optogenetics
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
In the presence of Cre, nEF-driven ChRmine-oScarlet expression (repressed in the presence of Flp). The purity of these AAV preparations is suitable for injection into animals.
Tracer Type
Optogenetics
Functional Gene
ChRmine
Serotype
AAV8
Virus Type
Adeno-Associated Virus (AAV)
Applications
Optogenetics
Sub Type
AAV8
Research Areas
Neural Circuit Mapping
Promoter
nEF
Key Components
AAV2-nEF-WPRE
Expression
Normal Expression
Properites
Titer
≥ 1×10¹³ vg/mL
Fluorophore
oScarlet
Shipping
Viral particles are shipped frozen on dry ice.
Handling Advice
The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-63 handling depending on the insert.
Storage
Store at -80°C. Thaw just before use and keep on ice.
Quality Control
Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.
Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.
Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.
Research Use Only
For research use only.
Gene/Insert
Gene/Insert Name
Con/Foff 2.0-ChRmine-oScarlet
Species
Prokaryotic
Insert Size (bp)
2500
Growth in Bacteria
Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
High Copy
Cloning Information
Cloning method
Restriction Enzyme
5′ cloning site
BamHI (not destroyed)
3′ cloning site
EcoRI (not destroyed)
5′ sequencing primer
GACCCTGCTTGCTCAACTCT
3′ sequencing primer
GGGCCACAACTCCTCATAAA
References
References
Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 10.1016/j.neuron.2020.06.003.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
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