Parkin Mitochondrial Recruitment Assay Cell Line

[CAT#: NCL2110P248]

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: PARK2-FP602+MTS-tGFP U2OS Stable Cell line (Parkinson's disease in vitro model)

Features

PARK2-FP062+MTS-tGFP cell line is obtained by double transfection of expression vector containing PARK2 protein fused with FP602 red fluorescent protein.

Neurological Disease Models

PD-related Cells

Cell Types

Other Cells

Relevant Diseases

CNS; Parkinson's Disease

Assay

Parkin Mitochondrial Recruitment Assay

Assay Description

The Parkin mitochondrial recruitment assay from Creative Biolabs is based on changes in the localization of two fluorescent proteins; Parkin fluorescent fusion peptide, which changes its localization from the cytoplasm to the outer mitochondrial membrane (OMM); and other fluorescent fusion peptides (MTS-tGFP), which directly interact with OMM combination allows mitochondrial integrity analysis.

Assay Characteristics

Readout: Parkin recruitment by mitochondria
Agonist: Proscillaridin A
EC50 Agonist: 2.59 × 10⁶-8 M
Type of assay: Cell based / Functional
Detection Method: Image Analysis
Pathway: PARKIN mitochondrial recruitment in Parkison´s disease
HCS Application: PARK2-FP602 redistribution quantification
Material provided: stable Cell Line (2 vial of cells); Division Arrested cells (2 million cells per vial)

Species

Human

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.