PRV-CMV-EGFP

[CAT#: NTA-2011-ZP11] Review(4) Q&As(3)

Retrograde, multisynaptic

Tracer Type:: Retrograde tracing virus

Applications:: Neural Tracing

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Product Overview

Description

Pseudorabies virus (PRV) has a double-stranded DNA genome with a genome of approximately 150 kb, which encodes capsid, outer skin, and envelope proteins. The reverse-transcribed anti-synaptic PRV is modified from the vaccine strain Bartha and can be used to analyze the connection structure of the brain center and peripheral neurons. After the virus infects nerve cells, it can replicate in the cell and express the target gene. The offspring virus is transported to the synapse, and then retrogrades the counter-synaptic label of the upstream neuron for further replication and transmission across the synapse.

After years of research, Creative Biolabs has established a stable production platform and can provide pseudorabies virus (PRV) services to promote PRV-based neuron tracking research.

Application field
●Multi-synaptic tracking from the periphery to the center of the brain or from higher to lower brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Retrograde tracing virus

Virus Type

Pseudorabies Virus (PRV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Retrograde

Synaptic Restriction

Polysynaptic

Cell Types

Neuron

Promoter

CMV

Expression

Normal Expression

Properites

Fluorophore

EGFP

Storage

Please refer to the protocal.

Research Use Only

For research use only.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

This tool has significantly enhanced our ability to map neural circuits efficiently

The expression of EGFP was robust and easily detectable under fluorescence microscopy, providing clear and distinct labeling of neuronal pathways. The virus exhibited excellent infectivity without significant toxicity to the host cells, allowing for extended observation periods. Additionally, the product's packaging and instructions were clear, making the application straightforward.

Excellent

We highly recommend it to other researchers in the field of neurobiology

The virus was easy to handle, and the infection process was smooth with minimal cell death observed. This product has provided us with consistent and reproducible results, which is crucial for our studies on synaptic plasticity.

Excellent

We appreciated the minimal off-target effects and the specificity of the neuronal labeling

In our recent study on neural pathways in rats, we employed PRV-CMV-EGFP, and the outcomes were outstanding. The fluorescence was bright and stable, making it easy to trace long-range projections. The instructions provided were comprehensive, and the virus performed as described.

Excellent

The results were highly satisfactory

Our lab has tried various tracers in the past, but this product stands out due to its reliability and high-quality labeling. We are very pleased with our purchase.

Q&As

Can PRV-CMV-EGFP be used in conjunction with other fluorescent markers?

Yes, PRV-CMV-EGFP can be used alongside other fluorescent markers. Researchers often use multiple fluorescent proteins to label different types of cells or neural pathways simultaneously. It's important to select compatible fluorophores with distinct emission spectra to avoid overlap and ensure clear visualization of each marker. This multi-color approach provides a more comprehensive view of neural networks and interactions.

How long does it typically take for the PRV-CMV-EGFP to express EGFP in neurons after injection?

The expression of EGFP from PRV-CMV-EGFP typically begins within 24 to 48 hours after injection, but full expression and visualization of neural pathways may take several days. The exact timeline can vary depending on factors such as the viral load, the health of the tissue, and the efficiency of transduction. Regular imaging over time can help monitor the progression of expression and optimize experimental conditions.

What kind of imaging equipment is recommended for observing PRV-CMV-EGFP expression?

For observing PRV-CMV-EGFP expression, a fluorescence microscope or confocal microscope equipped with appropriate filters for EGFP is recommended. These instruments allow for high-resolution imaging of fluorescent signals and detailed analysis of neural pathways. Confocal microscopy, in particular, provides superior resolution and the ability to perform optical sectioning, which enhances the clarity of 3D neural tracings.