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Creative Biolabs

rAAV-TPH2-CRE-WPRE-hGH polyA

[CAT#: NTA-2010-TT304] Review(4) Q&As(3)

Tracer Type:
Recombinase
Applications:
Recombinase

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Product Overview

Tracer Type

Recombinase

Functional Gene

TPH2

Serotype

The available serotypes are 1, 2, 5, 6, 8, 9, rh10, DJ, DJ8, PHP.B, PHP.eB, PHP.S, retro, anc80Novel peptide modified serotypes can also be produced on a case-by-case basis.

Virus Type

Adeno-Associated Virus (AAV)

Applications

Recombinase

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde/Retrograde

Cell Types

Neuron

Promoter

TPH2

Expression

Normal Expression
Properites

Titer

≥2×10^12vg/ml

Storage

Should be stored at -80°C

Research Use Only

For research use only.
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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
We used the product in our study on serotonin-producing neurons
The specificity of the TPH2 promoter in targeting these neurons was exceptional. The efficiency of CRE recombinase activity was also impressive, resulting in precise genetic modifications. This vector greatly facilitated our research, and we were able to achieve significant results with minimal off-target effects. The packaging and delivery were timely and well-handled.
user
Excellent
This product contributed significantly to our research progress
We recently incorporated the product into our experiments on neural circuitry. The vector's targeting accuracy and high transduction efficiency were impressive. Our team appreciated the high-quality packaging and prompt delivery.
user
Excellent
The vector demonstrated excellent targeting specificity and efficient CRE recombinase activity
We were able to achieve targeted genetic modifications without encountering significant off-target effects. The vector's performance in both in vitro and in vivo experiments was highly reliable, making it an invaluable tool for our research.
user
Excellent
We observed consistent and reproducible results across multiple experiments
The vector's specificity for TPH2-expressing neurons was remarkable, and the CRE recombinase activity was highly efficient. The product's packaging and shipping were excellent, ensuring the vector arrived in perfect condition. This is a top-notch product for neuroscience research.
Q&As
How can the efficiency of recombination be assessed using this vector?
The efficiency of recombination can be assessed through various methods, including PCR analysis to detect recombination events, immunohistochemistry to visualize recombined cells, or reporter assays if a reporter gene is included in the experimental setup. Monitoring the expression of the transgene or any downstream effects can also provide insights into the recombination efficiency.
Can this vector be used in combination with other AAV vectors or experimental tools?
Yes, rAAV-TPH2-CRE-WPRE-hGH polyA can be used in combination with other AAV vectors or experimental tools. For example, it can be used alongside AAV vectors expressing reporter genes or other regulatory elements to study gene function or neuronal circuitry. Combining vectors allows for more complex experimental designs and a deeper understanding of genetic and cellular interactions.
What is the primary application of rAAV-TPH2-CRE-WPRE-hGH polyA in neuroscience research?
The rAAV-TPH2-CRE-WPRE-hGH polyA is primarily used for genetic manipulation in neuroscience research. It allows for targeted recombination in serotonin-producing neurons (TPH2-expressing cells). This tool is invaluable for studying gene function and neuronal circuits in animal models, enabling researchers to investigate the role of specific genes in neurological processes and disorders.
For Research Use Only. Not For Clinical Use.
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