Creative Biolabs

Rat 9L/lacZ Cells

[CAT#: NCB2108FY0078]

Animal cells

Species:
Rat
Applications:
3D Cell Culture
Cell Types:
Glial Cell

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Description
Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.
Cell Types
Glial Cell
Applications
3D Cell Culture
Application Notes
This cell line is one of few models that permit quantitative analysis of microscopic tumor in the brain.
The tumor mimics important features of human brain tumor growth and spread.
Relevant Diseases
Gliosarcoma
Research Areas
Neural Cell
Species
Rat
Expression Profile
beta galactosidase (beta-gal)
Form
Frozen
Growth Pattern
Adherent
Morphology
Fibroblast
Culture Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%
Freeze Medium
Complete growth medium supplemented with 5% (v/v) DMSO
Tissue Source
Brain
Shipping
Dry ice
Storage
Vapor phase of liquid nitrogen
Handling Advice
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Research Use Only
For research use only
Additional Donor Information
The 9L/lacZ cell line was developed from the 9L cell line (a rat nitrosourea-induced glioma cell line) in 1989.
The 9L cells were infected with the BAG replication-deficient retroviral vector, which carried the E. coli lacZ gene encoding β-gal and the Tn5 neomycin gene, conferring resistance to G418.
For Research Use Only. Not For Clinical Use.
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