Creative Biolabs

Rat astrocytes (RA), Cryopreserved, Neonatal

[CAT#: NCL-2103-P11]

Positive for GFAP

Species:
Rat
Applications:
Cell Assay
Cell Types:
Astrocyte

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Description
Rat astrocytes (RA) are derived from the rat cerebral cortex. Astrocytes are the most abundant cells in the central nervous system, where they perform many functions, such as providing mechanical support and nutrition to neurons and removing waste from neurons. Provide signals to endothelial cells; regulate neurogenesis and control synaptic function. With increasing awareness of the importance of astrocytes in the nervous system, RA has become a useful in vitro model for exploring the functional diversity of astrocytes.
Cell Types
Astrocyte
Applications
Cell Assay
Application Notes
• Assess neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke
• Investigate the effects of astrocytes on brain microvascular endothelial cells in relation to iron transport pathway
Species
Rat
Tissue Source
Normal healthy Rat brain
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
For Research Use Only. Not For Clinical Use.
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