Creative Biolabs

Recombinant Human APOE3 (Lys19-His307) Protein, Carrier Free

[CAT#: NPP2010135LS]

Human APOE3 Recombinant Protein Product

Species:
Human
Tag:
N-His

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Description
ApoE is an approximately 34-kD protein that aids in the transport of cholesterol and other lipids in plasma and the central nervous system (CNS). ApoE is comprised of two independently folded N-terminal and C-terminal domains which are joined together by a flexible hinge region. The N-terminal domain contains the ApoE receptor binding region, whereas the lipid binding region is found within the C-terminal domain. ApoE regulates lipid homeostasis by mediating lipid transport from one tissue or cell type to another. More specifically, ApoE regulates the clearance of apolipoproteins from plasma by functioning as the ligand for binding to specific receptors, including the LDL receptor family members and heparan sulfate proteoglycans (HSPGs). In the CNS, ApoE is mainly produced by astrocytes; transporting cholesterol to neurons via ApoE receptors, which are members of the low-density lipoprotein receptor (LDLR) family. ApoE is also produced by macrophages and liver cells, where it mediates cholesterol metabolism in peripheral tissues (in an isoform dependent manner). ApoE has three major isoforms: ApoE2, ApoE3, and ApoE4. Each ApoE isoform has effects on lipid and neuronal homeostasis. ApoE3, the most common of the three isoforms, is considered to be the normal form. ApoE2 and ApoE4 differ from ApoE3 by single amino acid substitutions at positions 112 or 158, respectively. The residues that distinguish the ApoE isoforms are located in the N-terminus which affects the ability of these isoforms to bind receptors. ApoE isoforms also differentially regulate Aβ production, aggregation and clearance. Perhaps most importantly, ApoE isoforms have been studied extensively in their relation to Alzheimer's disease. ApoE alleles and their asociated protein isoforms are a main genetic determinant of Alzheimer's diseases risk. In the context of Alzheimer's disease models, the ApoE4 isoform exhibits diminished neuroprotection and/or enhanced neurotoxicity when compared to the ApoE2 and ApoE3 isoforms.
Species
Human
Biological Activity
When human VLDLR is coated at 1.0 µg/mL (100 µL/well), human Apolipoprotein E3/APOE3 binds with an EC50 of 0.05 - 0.2 µg/mL. HRP anti-His tag antibody was used to detect the binding.
Expression Host
CHO
Nature
Recombinant
Construction
Lys19-His307
Accession Number
P02649
Molecule Mass
35.2 kD
N-terminal
Lys
Tag
N-His
Conjugation
Unconjugated
Relevant Diseases
Neurological Disorders
Application Notes
The optimal working dilutions shoµld be determined by the end user.
Research Areas
Neurodegeneration
Form
Liquid
Concentration
0.2 mg/mL
Formulation
0.22 µm filtered protein solution is in 20 mM Tris, 300 mM NaCl, 1 mM TCEP, pH 7.5
Carrier Protein
Carrier Free
Purification
Affinity Chromatography
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Endotoxin Level
Less than 1.0 EU per µg protein as determined by the LAL method.
Shipping
Product is shipped at ambient temperature. Upon receipt, store lyophilized powder at ≤ -20°C. Reconstituted peptide should be aliquoted and stored at ≤ -20°C. Do not freeze/thaw.
Storage
Unopened vial can be stored at -20°C or -70°C for six months. Avoid repeated freeze/thaw cycles.
Handling Advice
Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Apo E
Official Name
APOE
Full Name
Apolipoprotein E
Alternative Names
APOE; AD2; APO-E; LDLCQ5; LPG; apolipoprotein E; ApoE4
Gene ID
348 (Human); 11816 (Mouse)
Uniprot ID
P02649 (Human); P08226 (Mouse)
1. Schwall RH, et al. 1988. Mol. Endocrinol. 2:1237.
2. Hashimoto M, et al. 1992. J. Biol. Chem. 267:4999.
3. Shav-Tal Y and Zipori D. 2002. Stem Cells 20:493.
4. Dohi T et al. 2005. Gastroenterology 128:411.
5. Tsuchida K, et al. 2008. Endocr. J. 55:11.
6. Djouad F, et al. 2010. Steam Cell Res. Ther. 1:11.
7. Sako D, et al. 2010. J. Biol. Chem. 285:21037.
8. Jones CP, et al. 2012. J. Allergy Clin. Immunol. 129:1000.
Neural Proteins & Peptides
Neural Genes
Neural Antibodies
Kits
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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