Creative Biolabs

Recombinant Human BDNF (His129-Arg247) Protein for ELISA use

[CAT#: NPP2010138LS]

Human BDNF Recombinant Protein Product

Species:
Human
Tag:
C-Fc

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Description
Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammals examined. All neurotrophins are noncovalently linked homodimers and initially produced as proneurotrophins. These proneurotrophins have distinct biological activity different from their mature counterparts. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during the development. BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.
Species
Human
Expression Host
CHO
Nature
Recombinant
Construction
His129-Arg247
Accession Number
P23560
Molecule Mass
13.5 kD
N-terminal
His
Tag
C-Fc
Conjugation
Unconjugated
Relevant Diseases
Neurological Disorders
Application Notes
The optimal working dilutions shoµld be determined by the end user.
Research Areas
Neurodegeneration
Form
Liquid
Concentration
0.2 mg/mL
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Purification
Affinity Chromatography
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Shipping
Product is shipped at ambient temperature. Upon receipt, store lyophilized powder at ≤ -20°C. Reconstituted peptide should be aliquoted and stored at ≤ -20°C. Do not freeze/thaw.
Storage
Unopened vial can be stored at -20°C or -70°C for six months. Avoid repeated freeze/thaw cycles.
Handling Advice
Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
BDNF
Official Name
BDNF
Full Name
Brain Derived Neurotrophic Factor
Alternative Names
Brain Derived Neurotrophic Factor; Neurotrophin; Abrineurin; Brain-Derived Neurotrophic Factor; ANON2; BULN2;
Gene ID
627 (Human); 12064 (Mouse); 24225 (Rat)
Uniprot ID
P23560 (Human); P21237 (Mouse); P23363 (Rat)
1. Freeman GJ, et al. 1993. Science 262:909.
2. Chen C, et al. 1994. J Immunol. 152:4929.
3. Lanier LL, et al. 1995. J Immunol. 154:97.
4. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.
5. Rudd CE, et al. 2009. Immunol Rev. 229:12.
6. Wing K, et al. 2011. Trends Immunol. 32:428.
Neural Proteins & Peptides
Kits
Neural Antibodies
Biomaterials
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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