Recombinant Mouse IL-1 Beta Protien
[CAT#: NPP2007FY61]
- Species:
- Mouse
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Recombinant mouse interleukin-1 beta. Interleukin-1 beta (IL-1 beta) is a 17.5 kDa pro-inflammatory cell-related cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1β promotes the proliferation of memory T cells and the production of cytokines. It weakens regulatory T cell functions and prevents CD4 + CD25- self-reactive effectors from being suppressed. IL-1β signals downstream of myc and is necessary for tumor cell angiogenesis and invasiveness. IL-1β directly participates in neuronal damage in neurodegenerative diseases, and stimulates mitotic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandins from synovial cells.
Species
Mouse
Biological Activity
The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.002ng/ml, corresponding to a specific activity of> 5x108 units/mg.
Expression Host
E. coli
Molecule Mass
17.5 kDa (153 amino acid residues)
Relevant Diseases
Amyotrophic Lateral Sclerosis; Frontotemporal Dementia
Applications
WB; FuncS; ELISA
Application Notes
WB: 0.2-0.4ng/well
ELISA: 1.5-3.0ng/lane
ELISA: 1.5-3.0ng/lane
Form
Lyophilised
Concentration
0.1 mg/ml after reconstitution
Carrier Protein
BSA free
Purity
>98% by SDS PAGE and HPLC analysis
Endotoxin Level
< 0.1 ng/ug
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend micr°Centrifugation before use.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend micr°Centrifugation before use.
Handling Advice
Reconstitute with 0.1 ml distilled water.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
1. Allan, S. et al. (2005) Interleukin-1 and neuronal injury.
Neural Vectors
Neural Proteins & Peptides
Neural Antibodies
For Research Use Only. Not For Clinical Use.
