Creative Biolabs

Recombinant Mouse IL-1β (Val118-Ser269) Protein, Carrier Free

[CAT#: NPP201090LS]

Mouse IL-1β Recombinant Protein Product

Species:
Mouse
Tag:
C-His

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Description
Interleukin-1 is a key mediator of inflammation, with pleiotropic effects on several cells and signaling pathways. The activity defined as IL-1 reflects the function of 2 molecules, IL-1α and IL-1β. IL1A encodes IL-1α, which is cell-bound, and IL1B encodes IL-1β, a secreted cytokine. IL-1α and IL-1β are synthesized as 31-kD precursors and are processed by proteases to their mature 17-kD forms. IL-1β-converting enzyme cleaves the inactive IL-1β Precursor and ProIL-1α is processed by calpain. IL-1β is a tumor-promoting cytokine, and it enhances tumor metastasis and angiogenesis. IL-1β is able to facilitate tumor progression in murine models of lung cancer. Upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor.
Species
Mouse
Biological Activity
Mouse IL-1β induces the proliferation of D10.G4.1 mouse helper lymphocytes. The ED50 = 2 - 10 pg/ml.
Expression Host
E. coli
Nature
Recombinant
Construction
Val118-Ser269
Accession Number
NM_008361
Molecule Mass
18.0 kD
N-terminal
Val
Tag
C-His
Conjugation
Unconjugated
Relevant Diseases
Neurological Disorders
Applications
ELISA
Application Notes
The optimal working dilutions shoµld be determined by the end user.
Research Areas
Neuroinflammation
Form
Liquid
Concentration
0.2 mg/ml
Formulation
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Carrier Protein
Carrier Free
Purification
Affinity Chromatography
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Shipping
Product is shipped at ambient temperature. Upon receipt, store lyophilized powder at ≤ -20°C. Reconstituted peptide should be aliquoted and stored at ≤ -20°C. Do not freeze/thaw.
Storage
Unopened vial can be stored at -20°C or -70°C for six months. Avoid repeated freeze/thaw cycles.
Handling Advice
Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Il-1β
Official Name
IL1B
Full Name
Interleukin 1 beta
Alternative Names
IL1B; IL-1; IL1-BETA; IL1F2; interleukin 1 beta; IL1beta
Gene ID
16176(Human)
Uniprot ID
P10749(Human)
1. Soo DS and Roby KF, et al. Mol Endocri 20:2999-3013 2006.
2. Yatabe T, et al. Ann Rheum Dis published online 28 Jul 2008.
3. Ledford H et al, Nature 450:29-30 2007.
4. Boraschi B Tagliabue A et al, Vitam Horm 74:229-254 2006.
Neural Genes
Neural Antibodies
Neural Proteins & Peptides
For Research Use Only. Not For Clinical Use.
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