Creative Biolabs

Recombinant Mouse IL-1β (Val118-Ser269) Protein for ELISA use

[CAT#: NPP201091LS]

Mouse IL-1β Recombinant Protein Product

Species:
Mouse
Tag:
C-His

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Bulk Quote
Description
Interleukin-1 is a key mediator of inflammation, with pleiotropic effects on several cells and signaling pathways. The activity defined as IL-1 reflects the function of 2 molecules, IL-1α and IL-1β. IL1A encodes IL-1α, which is cell-bound, and IL1B encodes IL-1β, a secreted cytokine. IL-1α and IL-1β are synthesized as 31-kD precursors and are processed by proteases to their mature 17-kD forms. IL-1β-converting enzyme cleaves the inactive IL-1β Precursor and ProIL-1α is processed by calpain. IL-1β is a tumor-promoting cytokine, and it enhances tumor metastasis and angiogenesis. IL-1β is able to facilitate tumor progression in murine models of lung cancer. Upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor.
Species
Mouse
Expression Host
E. coli
Nature
Recombinant
Construction
Val118-Ser269
Accession Number
NM_008361
Molecule Mass
18.0 kD
N-terminal
Val
Tag
C-His
Conjugation
Unconjugated
Relevant Diseases
Neurological Disorders
Application Notes
This IL-1β protein is biologically active, and can be used for in vitro assays
Research Areas
Neuroinflammation
Form
Liquid
Concentration
Batch Dependent
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Purification
Affinity Chromatography
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Shipping
Product is shipped at ambient temperature. Upon receipt, store lyophilized powder at ≤ -20°C. Reconstituted peptide should be aliquoted and stored at ≤ -20°C. Do not freeze/thaw.
Storage
Unopened vial can be stored at -20°C or -70°C for six months. Avoid repeated freeze/thaw cycles.
Handling Advice
Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Il-1β
Official Name
IL1B
Full Name
Interleukin 1 beta
Alternative Names
IL1B; IL-1; IL1-BETA; IL1F2; interleukin 1 beta; IL1beta
Gene ID
16176(Human)
Uniprot ID
P10749(Human)
1. Bonsall D, et al. 2015. PLoS One. 10: e0140678. PubMed
2. Zeng J, et al. 2019. Cell Rep. 27:549. PubMed
3. Cai Y, et al. 2019. Cell Rep. 27:3034
Neural Genes
Neural Proteins & Peptides
Neural Antibodies
For Research Use Only. Not For Clinical Use.
Send Inquiry Send Inquiry
Inquiry Basket
compare

Go to compare