RV-N2C(G)-△G-dsRed [Rabies Virus (RABV)]
[CAT#: NTA-2011-ZP9]
Combined with AAV-RVG, Retrograde, Monosynaptic
- Tracer Type:
- Virus Vector/Partical
- Applications:
- Neural Tracing
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
Rabies virus (RABV) is an enveloped, bullet-shaped, negative single-stranded RNA virus of the genus Rabies virus of the Rhabdoviridae family. The RNA genome of the virus is approximately 12 kb, which encodes 5 viral proteins, including nucleoprotein (N), Phosphoprotein (P), matrix protein (M), glycoprotein (G) and viral RNA polymerase (L) from the 3'end to the 5'end.
Through reverse genetics, a recombinant RABV constructed by Wickersham et al. Based on the SAD-B27 vaccine strain. The glycoprotein gene of modified RABV is completely removed (G deletion) and loses the ability to cross synapses, but its replication and transcription are not affected. RABV-∆G can be used to reverse high-brightness and finely labeled neurons. If we provide G protein first (inject AAV-G first), then the recombinant RABV will have the ability to propagate retrograde in the synapse.
In order to further improve the targeting effect of G-deficient RABV on specific starting cells, the virus was modified by binding to the envelope protein EnvA of avian sarcoma and leukemia virus (ASLV). The infectivity of these viruses is limited to cells that carry TVA receptors. Of course, the receptor TVA and EnvA proteins must be provided first.
After years of research, Creative Biolabs has established a stable production platform and can provide rabies virus (RABV) services to promote RABV-based neuron tracking research.
Application field
●Changes in neural networks during neural development
●Single synapse tracking of specific types of neurons
Through reverse genetics, a recombinant RABV constructed by Wickersham et al. Based on the SAD-B27 vaccine strain. The glycoprotein gene of modified RABV is completely removed (G deletion) and loses the ability to cross synapses, but its replication and transcription are not affected. RABV-∆G can be used to reverse high-brightness and finely labeled neurons. If we provide G protein first (inject AAV-G first), then the recombinant RABV will have the ability to propagate retrograde in the synapse.
In order to further improve the targeting effect of G-deficient RABV on specific starting cells, the virus was modified by binding to the envelope protein EnvA of avian sarcoma and leukemia virus (ASLV). The infectivity of these viruses is limited to cells that carry TVA receptors. Of course, the receptor TVA and EnvA proteins must be provided first.
After years of research, Creative Biolabs has established a stable production platform and can provide rabies virus (RABV) services to promote RABV-based neuron tracking research.
Application field
●Changes in neural networks during neural development
●Single synapse tracking of specific types of neurons
Tracer Type
Virus Vector/Partical
Virus Type
Rabies Virus (RABV)
Applications
Neural Tracing
Research Areas
Neural Circuit Mapping
Direction of Transport
Retrograde
Synaptic Restriction
Monosynaptic
Cell Types
Neuron
Expression
Normal Expression
Properites
Fluorophore
dsRed
Storage
Please refer to the protocal.
Research Use Only
For research use only.
References
References
1. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. 2000 Sep;81(Pt 9):2147-53.
2. Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013 Aug;8(8):1583-601.
3. Callaway EM, Luo L. Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses. J Neurosci. 2015 Jun 17;35(24):8979-85.
2. Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013 Aug;8(8):1583-601.
3. Callaway EM, Luo L. Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses. J Neurosci. 2015 Jun 17;35(24):8979-85.
Neural Tracers
For Research Use Only. Not For Clinical Use.
