Spared Nerve Injury (SNI) Model Development Service

Are you currently facing challenges in creating a reliable and reproducible animal model for neuropathic pain research? Our spared nerve injury (SNI) models development service helps you accelerate your drug discovery and validation efforts by providing a highly consistent and clinically translatable preclinical model. We achieve this through our advanced surgical techniques and comprehensive behavioral and molecular analysis platforms, delivering the high-quality data you need to make confident decisions.

Neuropathic pain is a chronic condition caused by damage to the somatosensory nervous system. The SNI model is a gold standard for studying this condition, offering a highly controlled and clinically relevant approach. It was developed to overcome the limitations of earlier, more variable models by creating a consistent and persistent pain phenotype. The model's validity is supported by extensive published data demonstrating its ability to replicate key human neuropathic pain symptoms, making it an invaluable tool for translational research.

How Our SNI Models Development Service Can Assist Your Project

At Creative Biolabs, our service is designed to be a true partnership, providing not just a model, but a complete solution to your research questions. We offer a full spectrum of services from initial study design to final data analysis, ensuring every step is executed with precision. You can expect to receive robust, high-quality data that directly addresses your project's goals, from validating a novel analgesic compound to uncovering a new molecular mechanism of pain. Our tailored approach minimizes experimental variability and maximizes the translatability of your results, giving you a competitive edge in the complex field of neuropathic pain research.

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Workflow: Our Path to Precision and Reproducibility

To ensure a seamless and efficient process, our workflow is designed for clarity and scientific rigor.

• Required Starting Materials:
  1. A detailed project proposal or research plan outlining your objectives.
  2. Your compound of interest, if applicable, with required concentration, route of administration, and dosing schedule.
  3. Relevant background literature or specific genes/proteins of interest for molecular analysis.

• Final Deliverables:
  1. A comprehensive project report including methodology, results, statistical analysis, and conclusions.
  2. Raw data files from all behavioral and molecular assays.
  3. High-resolution images from histology and Western blot analysis, if requested.
• Estimated Timeframe:

The typical timeframe for a standard SNI study ranges from 4 to 8 weeks, depending on the complexity of the study design, number of compounds, and the specific endpoints chosen. This includes time for surgical recovery, phenotype development, and the duration of your therapeutic intervention.

Why Choose Us?

At CBL, we don't just execute a protocol; we deliver a scientific partnership grounded in decades of expertise. Our SNI models development service stands out due to our rigorous standards for surgical precision and our holistic approach to data collection. We ensure the highest level of reproducibility, which is the cornerstone of reliable preclinical research. Our commitment to excellence is not just a promise—it's a proven track record.

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What Our Clients Say

• "Using CBL's SNI service in our research has significantly improved the consistency of our pain models. The detailed data and comprehensive reports allowed us to move a key compound into the next phase of our program with high confidence." - [March 2024], J. S*****
• "The level of surgical precision and post-operative care provided by CBL is unmatched. We were able to clearly differentiate our compound's effect from vehicle control, which was a challenge with our previous in-house efforts." - [January 2024], L. H*****
• "The team's expertise in both behavioral and molecular analysis, including qPCR and Western blot, provided a complete picture of our drug's mechanism of action. This holistic approach facilitated a deeper understanding of our research findings." - [May 2024], A. M*****

Spared Nerve Injury (SNI) Models

The SNI model is a sophisticated experimental technique designed to induce a reliable neuropathic pain state in laboratory animals. The procedure involves a highly precise surgical intervention where two of the three terminal branches of the sciatic nerve—the common peroneal and tibial nerves—are tightly ligated and then transected. Crucially, the third branch, the sural nerve, is left completely intact. This "spared" nerve is what gives the model its name and its unique advantage. Although uninjured, the sural nerve's receptive field becomes highly sensitized due to the nearby injury, leading to the development of robust and long-lasting mechanical allodynia and thermal hyperalgesia. This controlled partial denervation provides a more accurate representation of clinical neuropathic pain than models that involve complete nerve transection. The SNI model's reproducibility and its ability to dissect the complex interplay between injured and uninjured nerves make it the preferred choice for researchers investigating the underlying mechanisms of chronic pain and evaluating the efficacy of new therapeutic interventions.

What We Can Offer

Our SNI models development service at CBL provides a comprehensive and tailored approach to your preclinical pain research. We offer:

• Customized Study Design: We collaborate closely with your team to design a bespoke protocol that precisely meets your research goals, from species and cohort size to a full suite of behavioral and molecular endpoints.
• Expert Surgical Execution: Our highly skilled surgeons perform the SNI procedure with unparalleled precision and consistency, ensuring the highest level of reproducibility in every model.
• Comprehensive Phenotype Characterization: We provide robust and reliable data through a full range of behavioral assessments, including von Frey and hot plate tests, as well as advanced gait analysis systems to confirm and quantify the pain phenotype.
• Integrated Molecular Analysis: We go beyond behavioral endpoints to offer in-depth mechanistic insights, including histological staining for tissue analysis, and molecular techniques like Western blot and qPCR for gene and protein expression.
• End-to-End Service: CBL offers a one-stop solution, managing your project from the initial consultation and surgical modeling through to comprehensive data analysis and a clear, publication-ready final report.

Related Services

Our SNI models development service is often a critical part of a larger research effort. To support your project from start to finish, we also offer the following complementary services:

• Acute Pain Models
• Inflammatory Pain Models
• Biomarker Development & Validation
• Neuroinflammation Studies

Our SNI models development service is often a critical part of a larger research effort. To support your project from start to finish, we also offer the following complementary services.

Contact Our Team for More Information and to Discuss Your Project.