NSCs Differentiation Assay

Neural stem cells (NSCs) derived from neural tissue maintain stable biological properties, including differentiation capacity and histocompatibility, which have been applied in various aspects of research, treatment, and drug screening of neurological diseases. Creative Biolabs provides the most comprehensive NSCs differentiation assay to assist clients worldwide in research related to neurology.

NSCs Differentiation

Specialized stem cells NSCs can be encouraged to differentiate by particular circumstances since they retain the ability to continuously self-renew and undergo many differentiations. NSCs undergo directionally directed differentiation into a variety of neuronal cell types, including different types of neurons and glial cells, which are extensively employed in scientific applications including testing for neurotoxicity and looking for neuroprotective agents, among others. For instance, various neurons such as glutamatergic, GABAergic, dopaminergic, motor, and sensory neurons that respond to capsaicin are differentiated by neuronal-restricted progenitors. Additionally, glial-restricted progenitors can be stimulated to differentiate into astrocyte and oligodendrocyte cells, which can be used as a cell treatment for amyotrophic lateral sclerosis.

NSCs Induced Differentiation Platform

Induced pluripotent stem cells are directedly differentiated, and then pure neural rosettes are harvested to produce NSCs. Then, brain progenitor cells are grown in high numbers on the monolayer and stimulated to differentiate. With the help of a steady method, it is possible to produce many different kinds of neurons in huge numbers, each batch of which will have the same level of quality neurons. Using flow cytometry sorting techniques or immunomagnetic beads with particular antibodies on the surface, neuronal cells expressing particular antigens can be isolated and differentiated under controlled conditions. Moreover, the choice of high-quality cell culture media and control of suitable growth and differentiation conditions are critical to increasing the survival rate of neural cells because differentiation of NSCs demands rigorously conditioned medium.

Fig.1 Identification of neural stem cell differentiation (Zhu, 2022)

Application of NSCs Differentiation in Neural Repair

Normal brain physiological processes are preserved by the regeneration of differentiated neurons and glial cells from NSCs. Recent research has established that the differentiation and involvement of NSCs in the neurodevelopment and healing of injured neural tissues are intimately related to potential therapeutic options for a range of neurological illnesses, including brain injury, neurodegenerative diseases, and depression. NSCs can act as vectors for gene treatment of intracranial cancers and neurological illnesses, bridging the lack of viral vectors, and can also be used to rebuild parts of the destroyed brain loops and functions through post-transplantation differentiation. To examine the regeneration and reconstruction of the central nervous system and to identify new targets for the treatment of brain-related illnesses, it is advantageous to analyze NSC differentiation.

NSCs Differentiation Assay

Since differentiation gives rise to neuronal cells expressing different markers, NSCs differentiation assay can be performed by immunofluorescence labeling and immunoblotting analysis to label the differentiated cells with spectrum-specific antibodies. Quantification of NSCs differentiation can be determined by counting of labeling positive cells. For example, Nestin-positive labeled progenitor cells, GFAP-positive labeled astrocytes, β-tubulin III-positive labeled neurons, and GalC/O4-positive labeled oligodendrocytes.

Fig.2 Differentiation of NSCs/NPCs. (Namekata, 2010)

Creative Biolabs aims to provide a high-quality service platform for NSCs differentiation assay. Our most professional scientific team guarantees to provide the most suitable solution for NSCs differentiation analysis to facilitate your neurological research. Please feel free to contact us to discuss your topic and you will get our response in the shortest time possible.

References

1. Zhu, Y.; et al. Deep learning-based predictive identification of neural stem cell differentiation. Nat Commun. 2021, 12(1): 2614.
2. Jiao, Q.; et al. Cell-Cell connection enhances proliferation and neuronal differentiation of rat embryonic neural stem/progenitor cells. Front Cell Neurosci. 2017, 11: 200.