Pluripotent Stem Cell Lines

Pluripotent stem cells are cells that have the ability to self-renew and can divide and develop into the three main germ cell layers of an early embryo, thereby developing all cells of the body, but without extra-embryonic tissues (such as the placenta). Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are the two main types of pluripotent stem cells. There are currently two main methods of generating cells with multipotent properties. The first involves the isolation of internal cell masses from early human blastocysts and cultivation under appropriate culture conditions to produce human embryonic stem cells (hESCs). The second involves artificial expression of key developmental transcription factors in somatic cell types, which can reprogram cells into induced pluripotent stem cells (iPSCs) under appropriate culture conditions.

Cytokines for iPSC Generation

Unlike ES cells, iPS cells are ideal for treatment and research, and isolating them will not cause the same moral issues. Human iPS cells may be an ideal source for patient-specific therapies because they can be derived from the patient itself. In addition, iPS cells can also serve as useful research tools in the following ways: 1) human disease models for screening new drugs or studying pathogenesis and toxicology mechanisms, 2) for screening potential teratogens or for research, and 3) for understanding the tissue repair and regeneration.

In order to generate iPS cells, Creative Biolabs provides multiple key cytokines which play an important role in early development, for example, OCT-3 / 4, KLF-4, SOX2, c-MYC, and NANOG, which have proved to be essential for the specification of pluripotent stem cell identity.

Culture Products for hPSCs

Creative Biolabs iPSC archive includes well-characterized human/mouse iPS cell lines suitable for multiple uses, such as genome editing for disease models, differentiation into selected cell lineages, and drug toxicity / efficiency screening:

• Footprint-free iPSC cells and retrovirus-reprogrammed iPSC
• Characterized by pluripotency markers, karyotype and differentiation potential
• Provide more characterization options
• Multiple donor tissue sources are available: PBMC, fibroblasts, umbilical cord blood, fat cells in health and sick patients
• Tested by CRISPR genome editing and differentiation potential
• Can be used to construct engineered and / or differentiated isogenic cell lineages

Creative Biolabs also provides a series of research tools needed to study the function of PSCs. Our product portfolio includes cell lines from multiple species, optimized serum-free media, reliable cell differentiation media, media additives, and key cell markers and research antibodies.

Please browse the options below to take a deeper look at the tools you need for your research.