Methods and Applications of Cerebrospinal Fluid Histologic Testing
Creative Biolabs describes in detail the various methods of cerebrospinal fluid histologic testing, including proteomics, metabolomics, genomics, and microbiomics, and describes their wide range of applications in diagnosing neurological disorders, therapeutic monitoring, and pathogenesis research. As your partner, we also offer a range of cerebrospinal fluid-related services.
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Cat. No | Product Name | Species |
NRZP-0822-ZP495 | NeuroBiologics™ Monkey Cerebrospinal Fluid | Monkey |
NRZP-0822-ZP496 | NeuroBiologics™ Rat Cerebrospinal Fluid | Rat |
NRZP-0822-ZP497 | NeuroBiologics™ Mouse Cerebrospinal Fluid | Mouse |
NRZP-0822-ZP498 | NeuroBiologics™ Pig Cerebrospinal Fluid | Pig |
NRZP-0822-ZP491 | NeuroBiologics™ Human Cerebrospinal Fluid | Human |
Major Methods of Cerebrospinal Fluid Histologic Testing
- Proteomics
In cerebrospinal fluid, proteomics testing methods include the following:
Methods | Descriptions | Advantages and Disadvantages |
Two-Dimensional Gel Electrophoresis (2-DE) | It first separates proteins according to the isoelectric point by Isoelectric Focusing (IEF), and then performs SDS-PAGE in the second direction to separate proteins according to their molecular weight. Protein expression profiles can be obtained by staining and image analysis. |
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LC-MS/MS | It first separates complex protein mixtures into individual components by liquid chromatography, and then identifies and quantifies the proteins by mass spectrometry. |
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Protein Chip | It immobilizes a large number of protein probes on the surface of the chip and then performs hybridization reactions with proteins in the sample. By detecting the hybridization signals, the expression levels of multiple proteins can be detected simultaneously. |
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- Metabolomics
Metabolomics is the study of the composition and changing law of metabolites in organisms. Cerebrospinal fluid metabolomics testing methods mainly include the following:
Methods | Descriptions | Advantages and Disadvantages |
Nuclear Magnetic Resonance (NMR) | NMR is a non-destructive analytical technique capable of detecting multiple metabolites simultaneously. It obtains information about metabolites by detecting the resonance signals of atomic nuclei in the metabolite. |
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GC-MS | GC-MS first separates the metabolites by gas chromatography, and then identifies and quantifies the metabolites by mass spectrometry. |
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LC-MS | It separates metabolites by liquid chromatography, and then identifies and quantifies the metabolites by mass spectrometry. |
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- Genomics
Genomics is the study of the structure and function of an organism's genome. Cerebrospinal fluid genomics testing methods mainly include the following:
Methods | Descriptions | Advantages and Disadvantages |
PCR | PCR is a technique used to amplify specific DNA fragments. By designing specific primers, it is possible to detect pathogenic DNA, gene mutations, etc. in cerebrospinal fluid. |
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Gene Chip | A large number of gene probes are immobilized on the surface of the chip and then hybridize with the DNA in the sample. By detecting the hybridization signals, the expression levels and mutations of multiple genes can be detected at the same time. |
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NGS | NGS is a massively parallel sequencing technology capable of sequencing a large number of DNA molecules at the same time. NGS includes whole genome sequencing, whole exome sequencing, and transcriptome sequencing. In cerebrospinal fluid, NGS can be used to detect the genome of pathogens, gene mutations, and gene expression profiles. |
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- Microbiomics
Microbiomics is the study of the composition, structure and function of microbial communities. Cerebrospinal fluid microbiomics testing methods include the following:
Methods | Descriptions | Advantages and Disadvantages |
Culture Method | Culture method is the traditional microbial detection method, by inoculating cerebrospinal fluid onto culture medium, cultivating visible colonies, and then performing identification and drug sensitivity tests. |
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PCR | PCR can be used to detect microbial DNA in cerebrospinal fluid by designing specific primers. For example, 16S rRNA PCR is often used to detect bacteria, and ITS PCR is often used to detect fun | |
Metagenomic Sequencing | Metagenomic sequencing is the sequencing of the genomes of all microorganisms in an environmental sample. |
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Applications of Cerebrospinal Fluid Histologic Testing
- Research on the pathogenesis of neurological diseases
- Through the study of cerebrospinal fluid proteomics and metabolomics, we can gain insight into the pathogenesis of neurodegenerative diseases, such as protein aggregation, impaired energy metabolism, oxidative stress and so on.
- Cerebrospinal fluid genomics and microbiomics studies can help reveal the immunoregulatory mechanisms of neurologic autoimmune diseases and the role of microbial infections in their pathogenesis.
- Diagnosis of diseases of the nervous system
- Therapeutic monitoring of neurological diseases
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