Human Retinal Epithelial Cell ARPE-19

[CAT#: NCL2110P069]

Species:: Human

Applications:: Cell Culture

Cell Types:: Visual system-related Cells

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Product Overview

Description

Human retinal epithelial cells ARPE-19 were isolated from a 19-year-old male.

Features

Doubling time: 55 hours (from cell counting), 65 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154).

Cell Types

Visual system-related Cells

Applications

Cell Culture

Species

Human

Properties

Size

1x10^6

Form

Frozen

Culture Medium

90%DMEM+10%FBS+1% P/S

STR Analysis

Amelogenin X,Y
CSF1PO 11
D2S1338 19
D3S1358 14,15
D5S818 13
D6S1043 17,19
D7S820 9,11
D8S1179 13
D12S391 21,22
D13S317 11,12
D16S539 9,11
D18S51 12,16
D19S433 12,13
D21S11 28,29
FGA 23
Penta D 11,13
Penta E 7,11
TH01 6,9.3
TPOX 9,11
vWA 16,19

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.