3D Neural Spheroids Culture Technology
Creative Biolabs is always the leader in 3D neural spheroids culture services and offers a variety of technologies to help you grow 3D neural spheroids quickly and efficiently. In addition, we also provide functional tests and a one-stop service for your research and development to bring more physiological relevance.
3D Neural Spheroids
Although many studies have been focused on promoting nerve regeneration and functional recovery, there is still a lack of effective treatments for neurological diseases. This may be due to the lack of suitable models to simulate complex cell-cell and cell-environment interactions in vivo. The growth pattern, morphology, differentiation, and function of neural stem cells (NSCs) under two dimension (2D) culture are obviously different from those under in vivo physiological conditions. In order to make cell culture in vitro more close to the microenvironment of living conditions in vivo and obtain more consistent experimental results with in vivo experiments, the improved three dimension (3D) cell culture technology has been widely used in basic research and drug discovery.
Due to the possibility of contact differentiation in cell adherence culture, an undifferentiated state is a primary prerequisite for studying the characteristics of stem cells. Therefore, neural spheroids are considered as a suitable in vitro model for studying NSCs. Neural spheroids are multi-cellular aggregates of NSCs that spontaneously self-assemble or proliferate in vitro without scaffolds. 3D neural spheroids are similar to natural tissues both in morphology and function, so as to better simulate the growth of cells in vivo, more intuitively reflect the biological functions of NSCs, and more accurately construct the target neural tissue model. 3D neural spheroids can be formed by 3D suspension drop culture and ultra-low adhesion culture.
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3D Suspension Drop Culture
When a drop of cell suspension is added above the hole, the geometry of the 3D droplet culture plate guides the cell suspension through a small hole, resulting in the cell suspension becoming a droplet held by surface tension. This method requires a small amount of cell suspension (usually 20μL). The density of cells to be inoculated depends on the size of the neural spheroids required. Neural spheroids formation in the 3D suspension culture plate is divided into three processes: cell suspension distribution, suspension droplet formation, NSCs generate endogenous extracellular matrix and continuously aggregate to form neural spheroids. The drop inlet is located above each culture hole and can be used to change the medium and add exogenous extracellular matrix, growth factors, and small molecules.
Fig.1. Spheroid formation in hanging drop method. (Białkowska, 2020)
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Ultra-low Adhesion Culture
Ultra-low adhesion culture plane is a hydrophilic and uncharged plane covalently coupled with hydrogel to minimize cell attachment, protein absorption, and enzyme activity, and maintain the growth of NSCs in a suspended and non-adherent state. A single 3D neural spheroid of uniform and repeatable size can be generated by applying the package to each hole of the spheroid microplate. Neural spheroid can be easily analyzed by fluorescence staining, which is suitable for high-throughput screening and functional assay
Fig.2. Spheroid formation in a spheroid microplate. (Sherman, 2019)
Creative Biolabs can provide you with personalized 3D neural spheroids culture solutions to meet your experimental needs and purposes. Please contact us to discuss your project, and our professional team will provide you with the most professional laboratory services according to your needs.
References
- Białkowska, K.; et al. Spheroids as a type of three-dimensional cell cultures-examples of methods of preparation and the most important application. International Journal of Molecular Sciences. 2020, 21(17).
- Sherman, H.; et al. A novel three-dimensional glioma blood-brain barrier model for high-throughput testing of tumoricidal capability. Frontiers in Oncology. 2019, 9:351.
