How to Select Promoters and Serotypes in Neurological Research?

Currently, gene therapy has been applied to the treatment of a variety of diseases, utilizing viral vectors to deliver genetic material around the organ and achieve specific expression in the target cells, thus enabling gene therapy. In neuroscience research, especially in vitro experiments, adeno-associated viral tool vectors are usually utilized to study the functional roles of genes, so how can AAV be utilized more efficiently for gene transduction in the nervous system?

[Flyer]

Ready-to-Use AAV Biosensors

View

Creative Biolabs presents selection strategies for AAV in neurological research. As a partner, we offer the following related services to help accelerate the progress of your program.

Selection of AAV Serotypes

It has been reported that there are more than ten AAV serotypes applicable to the nervous system, such as 1, 2, 5, 6, 8, 9, PHP.B, PHP.eB, PHP.S, DJ, Retro, rh10, etc. Different serotypes have different infection efficiencies and spreading abilities in different parts of the nervous system, and the appropriate serotypes are related to the ability of the genes to achieve highly efficient and stable expression, and even related to the ultimate Therefore, it is important to select the appropriate AAV serotype by combining the labeled cells and the characteristics of each AAV serotype.

A number of AAV products are important tools commonly used in research. You can browse the table below to see a list of our recommended products.

Selection of Promoters

In addition to AAV vectors and serotypes, optimization of promoters and regulatory elements is also necessary. Currently, broad-spectrum type promoters used in the study, such as

• Cytomegalovirus promoter CMV and CMV enhancer fused chicken ß-actin promoter CAG can achieve high expression of target genes.
• The tetracycline promoter TRE acts as an inducible promoter and can initiate gene expression under specific conditions.

However, the composition of the nervous system is extremely complex, and sophisticated studies of the brain are critical, and it is particularly important to select a promoter with relatively higher specificity in order to achieve specific infection of a certain brain region or even a certain class of cells.

The following table shows some common neural tissue-specific promoters.

Therefore, to drive more efficient gene transduction in neurological studies, all of these factors can be taken into account to optimize the choice.

Related Scientific Resources

Applications of AAV and LV in Nervous System Research